FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n02_FluA_CM530203.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n02_FluA_CM530203.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2862737
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA96240.33618177289775486No Hit
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC64110.22394652390352307No Hit
GGACCAATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCAC46320.16180319742959273No Hit
GAGCAAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCAGAGAC45610.15932305342754155No Hit
TCCCACCTGCAGTCTGGATTCTGTCCCAATCACACACTAAAGAAGAGCCC41960.1465730173606587No Hit
ATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGGCA37160.1298058466425662No Hit
ACACAATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA36080.12603323323099538No Hit
CTTTCTCTAGACCCTGGACCCTGCATAAGATGCAATAAACATGGCTACAG35520.12407706331388459No Hit
GAACCAGTTAAGAGGCTGTTTTTGATCTGGGACAGAGAGAGGGTGATGAC34760.12142226128351993No Hit
GGATTGCAGTGGTACGATCTCAGCTCACTGCAGCCCCCACCTCCCATACT31590.11034894228844634No Hit
CTAGAAAAAGAAGGGGCAGCCTCTGGGAAGGGAGGCAAAGGCAGCCAGGT30640.10703043975049054No Hit
ACACGATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA29410.10273385225397931No Hit
AAACATGGCTACAGTCATGTTAAGGGCAGAGGCTCACTGGGGTAAGTGGC29250.10217494656337624No Hit
CTAGACAGTGTTGACAACCACCACCTGTGTTGTGATGAACTTCAACTTTT29120.10172083568976123No Hit
GAGCAAAAGCAGGACAGGCAAGGCTGGCAAGAAGGAACCAGTTAAGAGGC29100.10165097247843585No Hit
GAGGCAAGAGAGTCACTTGAGTATGGGAGGTGGGGGCTGCAGTGAGCTGA29020.1013715196331343No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA17500.092.388821
GAGCAAA78650.087.5744551
AGCAAAA87200.078.567912
CAAAAGC96000.071.365854
GCAAAAG96850.070.365223
AGCGAAA25450.062.6707232
AAAGCAG136500.061.76996
CGAAAGC26150.061.5475964
AAAAGCA114450.060.3046725
GCGAAAG27550.058.4199523
ATATCGT23950.057.517591
TATCGTG24250.056.803062
ATCGTGT24600.055.994883
TCGTGTA24600.055.994884
TACGACG2200.052.726283
AAGCAGG169750.049.8414767
TTACGAC2200.049.430892
ATTACGA2450.047.348531
CGACGGG2300.047.2817235
ACGACGG2600.044.6145444
Produced by FastQC (version 0.11.5)