FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n02_FluA_BHHS1178.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n02_FluA_BHHS1178.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2964214
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT40780.13757441264362152No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA38540.1300176033174393No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC37540.12664402772539363No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT37300.12583436958330269No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA35940.1212463067781206No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT34400.11605100036637031No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG34340.11584858583084756No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG33500.11301478233352923No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC33120.11173282360855188No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTG31250.10542423725142652No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT30210.10191571863569905No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA9950.080.15361
AGCGAAA19600.066.953921
CGAAAGC19500.066.922073
GCGAAAG24700.052.8332182
GCAAAAG59250.051.6363533
TAGACCG450.0096415648.332619
CAAAAGC77150.040.8775444
CAGTACG6700.036.7904939
AGCAAAA84400.036.5930522
GAGCAAA88800.035.843061
AAGCAGG112000.034.1133277
TTACGCC1501.1350221E-633.832825145
TGACGCC4900.031.070963145
AGGTCGT2351.2641976E-930.85066
CGACGAA18350.030.816975145
GTACTGA9950.029.8739246
TAGAAAC35150.028.875812
TCCAACT22700.028.744062
CAAGGGT28850.027.6425654
AAAAGCA120150.026.972135
Produced by FastQC (version 0.11.5)