FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n02_FluA_BHE1256.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n02_FluA_BHE1256.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3194951
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA120780.37803396671811246No Hit
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC112890.3533387523001135No Hit
GAGCAAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCAGAGAC85470.2675158398360413No Hit
GGACCAATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCAC85060.26623256506907306No Hit
TCCCACCTGCAGTCTGGATTCTGTCCCAATCACACACTAAAGAAGAGCCC69150.21643524423379262No Hit
ATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGGCA68940.2157779571580284No Hit
GAGGCAAGAGAGTCACTTGAGTATGGGAGGTGGGGGCTGCAGTGAGCTGA64900.20313300579570706No Hit
GAGCAAAAGCAGGACAGGCAAGGCTGGCAAGAAGGAACCAGTTAAGAGGC64460.2017558328750582No Hit
CTTTCTCTAGACCCTGGACCCTGCATAAGATGCAATAAACATGGCTACAG64120.20069165380001133No Hit
CTAGAAAAAGAAGGGGCAGCCTCTGGGAAGGGAGGCAAAGGCAGCCAGGT56760.1776553067637031No Hit
GTTAAGATACTTGTGGATAGTCGCACAGCTAGAAGATAACAGAGCTAGGA56490.17681022338057767No Hit
ACACAATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA54710.17123893292886183No Hit
GGATTGCAGTGGTACGATCTCAGCTCACTGCAGCCCCCACCTCCCATACT54360.17014345446925477No Hit
GAACCAGTTAAGAGGCTGTTTTTGATCTGGGACAGAGAGAGGGTGATGAC50830.15909477171950367No Hit
GAGTGAGACCCTGTCTCAAAAACAAGAAAGCTAAATTTGTGAACAGTTAA49770.15577703695612233No Hit
ACACGATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA44700.13990824898410023No Hit
GTCACGCAGTCGGCTCTGGTTCTCCTCCCACCTCTCTGACCATTCCTTCT43560.13634011914423727No Hit
GTCTAGAAGGAGTGTACAGAAAGAGAAAAGGATGGTTTGAGGACAGAGCC43350.13568283206847304No Hit
CTGCACTCCATCCTAGGAGACAGAGTGAGACCCTGTCTCAAAAACAAGAA41350.12942295515643276No Hit
CTAGACAGTGTTGACAACCACCACCTGTGTTGTGATGAACTTCAACTTTT40740.12751369269826046No Hit
TTGCATGCCCCTCTTCATTCCTCAGGGCTCTGTCCTCAAACCATCCTTTT37510.11740399148531541No Hit
TCCCCACAGGCATCTTGCCTCTCACCCTGTGCTGGGCCCCGTGCTCCAGA37110.11615201610290736No Hit
ATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCACACAAGT37030.11590162102642575No Hit
AGAAAAAGAAGGGGCAGCCTCTGGGAAGGGAGGCAAAGGCAGCCAGGTGC36770.11508783702786052No Hit
CACTCTCTAGAAGGAGTGTACAGAAAGAGAAAAGGATGGTTTGAGGACAG36530.11433665179841569No Hit
ATCTTGGACAGAGAGAGGGTGATGACTGATCTGGGGTTGGAGAAGAAAGC36440.11405495733737388No Hit
TCTTTAGTGTGTGATTGGGACAGAATCCAGACTGCAGGTGGGATGTGAGG35630.11151970718799756No Hit
CTCTCCACCTGCTTCAGCCCTGCCACTTACCCCAGTGAGCCTCTGCCCTT35400.11079982134311293No Hit
ATATGGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC35190.11014253426734871No Hit
GTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCCTAGCT34080.10666830258116634No Hit
GGCACGTGCCACTGTGCTCAGCTAATTTTTCTATTTTTAGTAGAGATGGG34050.10657440442748574No Hit
GGGACAGAGAGGGTGATGACTGATCTGGGGTTGGAGAAGAAAGCACATGT33880.10604231488996231No Hit
TAACATGACTGTAGCCATGTTTATTGCATCTTATGCAGGGTCCAGGGTCT33730.1055728241215593No Hit
GAGAGAGGGTGATGACTGATCTGGGGTTGGAGAAGAAAGCACATGTTTGA33230.10400785489354925No Hit
GTTCGAGACCAGTCTGGCCAACATGGTGAAACCCCATCTCTACTAAAAAT33180.10385135797074821No Hit
GCAGGCAGGATGTGAGGTTGCTCCCTCTCCACCTGCTTCAGCCCTGCCAC33130.10369486104794721No Hit
CCTTGCGTGGCCCATCTGCCGCTGCCCATGCCGCTGACGGCTTCTTCCTG33060.1034757653560258No Hit
CGATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGG33020.103350567817785No Hit
TATCTTAACTGTTCACAAATTTAGCTTTCTTGTTTTTGAGACAGGGTCTC32610.10206729305081676No Hit
ATCCACAAGTATCTTAACTGTTCACAAATTTAGCTTTCTTGTTTTTGAGA31960.10003283305440366No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA18200.0117.112231
GAGCAAA64100.0102.356831
CGCGTAG259.3896553E-486.997655
AGCAAAA85100.077.439162
CAAAAGC88100.074.555314
AGCGAAA29600.072.253112
AAAGCAG126750.068.7515956
GCGAAAG31400.067.880333
AAAAGCA99500.067.179095
GACGAAA5400.067.12782145
GCAAAAG98600.066.762913
CGAAAGC32550.065.482114
CGAATAA2400.063.435787145
TAGCCGC502.0111838E-457.998432145
AGCAGGA74350.049.729668
CACCATA44800.048.062322
TATCGTG53950.045.286082
ACCATAT48350.045.133223
ATCGTGT54000.045.1098943
ATATCGT54350.044.952791
Produced by FastQC (version 0.11.5)