FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n02_FluA_BHE1201.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n02_FluA_BHE1201.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3003903
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTTAGACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT36690.12214109443613858No Hit
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA31350.10436422214698676No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA12200.087.94921
CGAATAA3450.079.85361145
TACGACG1400.067.320193
GCAAAAG74550.066.3233953
CAAAAGC83100.060.1102034
GAGCAAA84050.059.9483341
AGCGAAA21900.059.9189871
CGAAAGC22450.058.4510423
AGCAAAA85150.058.322482
TTACGAC1700.055.440162
ACGACGG1750.053.856154
CGACGGG1800.052.3601465
ATTACGA1900.049.604351
GCGAAAG27250.048.155082
AAGCAGG135450.044.26467
AAAGCAG144550.041.7788936
TATCGTG12700.041.101612
ATATCGT12550.041.0151821
AAAAGCA126550.040.5030065
TCACGCA5550.039.188472
Produced by FastQC (version 0.11.5)