FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n02_FluA_BHE1163.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n02_FluA_BHE1163.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3388239
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA175580.5182042943251642No Hit
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC135670.40041449260220424No Hit
GGACCAATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCAC115770.34168191795206887No Hit
GAGCAAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCAGAGAC104540.30853785698116337No Hit
TCCCACCTGCAGTCTGGATTCTGTCCCAATCACACACTAAAGAAGAGCCC86190.25437993010528476No Hit
GAGGCAAGAGAGTCACTTGAGTATGGGAGGTGGGGGCTGCAGTGAGCTGA84210.24853618649687934No Hit
GGATTGCAGTGGTACGATCTCAGCTCACTGCAGCCCCCACCTCCCATACT77490.22870287485623064No Hit
ATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGGCA75120.22170809084010898No Hit
ATCTTGGACAGAGAGAGGGTGATGACTGATCTGGGGTTGGAGAAGAAAGC72970.2153626116693657No Hit
ACACAATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA71820.21196851815943324No Hit
AAACATGGCTACAGTCATGTTAAGGGCAGAGGCTCACTGGGGTAAGTGGC70750.2088105355023657No Hit
GAGCAAAAGCAGGACAGGCAAGGCTGGCAAGAAGGAACCAGTTAAGAGGC70490.20804317523055488No Hit
CTTTCTCTAGACCCTGGACCCTGCATAAGATGCAATAAACATGGCTACAG65580.19355187163597373No Hit
GAACCAGTTAAGAGGCTGTTTTTGATCTGGGACAGAGAGAGGGTGATGAC65430.1931091637868521No Hit
CTAGAAAAAGAAGGGGCAGCCTCTGGGAAGGGAGGCAAAGGCAGCCAGGT61390.1811855657171764No Hit
ACACGATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA56930.1680223856699601No Hit
GTCACGCAGTCGGCTCTGGTTCTCCTCCCACCTCTCTGACCATTCCTTCT56260.16604495727721688No Hit
CTGCACTCCATCCTAGGAGACAGAGTGAGACCCTGTCTCAAAAACAAGAA55520.16386093188821685No Hit
CTAGACAGTGTTGACAACCACCACCTGTGTTGTGATGAACTTCAACTTTT54380.16049635223489253No Hit
GTTAAGATACTTGTGGATAGTCGCACAGCTAGAAGATAACAGAGCTAGGA53230.1571022587249601No Hit
TTGCATGCCCCTCTTCATTCCTCAGGGCTCTGTCCTCAAACCATCCTTTT51230.15119948740333844No Hit
GCAGGCAGGATGTGAGGTTGCTCCCTCTCCACCTGCTTCAGCCCTGCCAC48480.14308317683610866No Hit
CACTCTCTAGAAGGAGTGTACAGAAAGAGAAAAGGATGGTTTGAGGACAG46750.13797727964290596No Hit
AGAAAAAGAAGGGGCAGCCTCTGGGAAGGGAGGCAAAGGCAGCCAGGTGC46150.13620644824641945No Hit
GGGACAGAGAGGGTGATGACTGATCTGGGGTTGGAGAAGAAAGCACATGT45010.13284186859309513No Hit
GTCTAGAAGGAGTGTACAGAAAGAGAAAAGGATGGTTTGAGGACAGAGCC43700.12897555337743294No Hit
TCCCCACAGGCATCTTGCCTCTCACCCTGTGCTGGGCCCCGTGCTCCAGA42720.12608319542983834No Hit
CCTTGCGTGGCCCATCTGCCGCTGCCCATGCCGCTGACGGCTTCTTCCTG41120.121360978372541No Hit
CTCTTTCCTCAGCCTCTTGAGTAGCTGGGACTACAGGCATGTGCCACTGT41030.12109535366306805No Hit
GTTCGAGACCAGTCTGGCCAACATGGTGAAACCCCATCTCTACTAAAAAT40410.11926549455336534No Hit
ATATTGTGTAGAATTAGACCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC39730.11725855230401397No Hit
CCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGG39640.11699292759454101No Hit
CGATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGG39600.11687487216810856No Hit
GGCACGTGCCACTGTGCTCAGCTAATTTTTCTATTTTTAGTAGAGATGGG39220.11575334561700046No Hit
GCCAACAGAACATGTGGGCAAAGCGCCGATGAGCTGTTCTGGAGCACGGG39200.11569431790378423No Hit
CCACACTGGCAAACCCTAAGGCCCCTTGCCAGTGCTCACCTTGCGTGGCC38990.11507452691501396No Hit
CATCTTCTGCTGGCAGAGTCTCCCCGATTCCATCTTCCACAGCCCTCTCA38730.11430716664320316No Hit
TTGCCAAACCCTAAGGCCTCTTGCCAGTGCTCACCTTGCGTGGCCCATCT37660.11114918398613556No Hit
CTCTCCACCTGCTTCAGCCCTGCCACTTACCCCAGTGAGCCTCTGCCCTT37170.10970300501233826No Hit
CTCTCTCACCCCACCTTCCTCTAGCATGCACCTGGCTGCCTTTGCCTCCC37110.10952592187268961No Hit
GACCCTTCCTTCTCAGGCTCCTTTGCGTGCCCCTCTTCATTCCTCAGGGC36830.10869953388766258No Hit
GAGAGAGGGTGATGACTGATCTGGGGTTGGAGAAGAAAGCACATGTTTGA36610.10805022904228422No Hit
ATATGGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC36520.10778460433281123No Hit
CCTCGTGTGGCCCATCTGCCGCTGCCCATGCCGCTGACGGCTTCTTCCTG35950.10610231450614907No Hit
ATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCACACAAGT35390.10444953853609501No Hit
GAGTGAGACCCTGTCTCAAAAACAAGAAAGCTAAATTTGTGAACAGTTAA35190.10385926140393284No Hit
TCTTTAGTGTGTGATTGGGACAGAATCCAGACTGCAGGTGGGATGTGAGG34670.10232454086031122No Hit
CCTTCTAGACAGTGTTGACAACCACCACCTGTGTTGTGATGAACTTCAAC34410.1015571805885004No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA17700.0116.738031
GAGCAAA78100.0108.7971041
AGCAAAA97950.086.3775252
CAAAAGC106800.079.491374
AGCGAAA27350.075.017652
AAAGCAG141950.074.2103656
GCAAAAG117450.072.345073
GCGAAAG28950.070.871593
AAAAGCA122750.069.693925
CGAAAGC29650.068.7093664
TACGACG4550.060.5489433
ACGACGG4550.060.5489434
CGACGGG4600.059.89085
TTACGAC4750.057.999512
ATTACGA4850.056.804481
CACCATA52800.053.5506822
TCGTGTA55850.050.756064
ATATCGT56400.050.5189321
ATCGTGT56300.050.479153
TATCGTG57200.049.938392
Produced by FastQC (version 0.11.5)