FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n02_FluA_BHE1039.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n02_FluA_BHE1039.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2937735
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA107010.3642602208844569No Hit
CCTCTTTCTTAATCCTTCCAGACTCGAAGTCAATTCTGGCATCAATCCGG70790.24096795660602469No Hit
GAGCAAAAGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCT65870.22422036024352093No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGACTCCAACACTGTGTCAAG59790.20352414360042684No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA51650.17581572197628445No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA49410.1681908000551445No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT49190.16744192379503256No Hit
GTATAGGAAACTTAAGAGAGAGATAACGTTCCATGGGGCCAAAGAAATAG46930.15974892221388245No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT45690.15552798329325143No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATATTATTTGGGTCTCCATTCCC45250.15403023077302752No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT43510.14810730035214204No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTG42670.14524795463171455No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA41130.14000582081093088No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG40620.13826978948067134No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA35850.1220327905682439No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC35810.12189663124822356No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC34880.11873092705775028No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG34050.11590562116732789No Hit
TTATTTGGGTCTCCATTCCCATTGAGGGCATTTTGGACAAAGCGTCTACG33140.1128079966368648No Hit
CTTTTAAGGCCATGTTTGAAGAGTCGATAGATGCATTTGAAAAAAAGACG32810.11168468224669685No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATATCAAAA32090.10923381448633045No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT31570.10746374332606583No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA31510.10725950434603529No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACCATCATTGCTTT31500.10722546451603021No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG31240.10634042893589789No Hit
AGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCTTCAACCC31110.10589791114583175No Hit
ATATACAGGAGAGTCGACGGGAAATGGATGAGGGAACTCGTCCTTTATGA30410.10351512304547551No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG29990.10208545018526177No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG29880.1017110120552058No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA14700.097.656951
CGAATAA2400.081.561584145
AGTAGAA14350.074.2712551
CAAAAGC84950.073.139264
GCAAAAG86150.071.615563
AGCGAAA22400.064.08412
CGAAAGC23100.061.2006154
AAGCAGG125950.060.382417
GCAGGGG11200.058.9055869
TAAGTAG753.7640348E-757.9993483
ACGGTCA400.006052318554.3743864
TCCGGTA400.006052318554.3743863
GCGAAAG27200.052.50863
TGACGCC2100.051.785133145
TATCGTG3250.051.3071172
AGCAGGG49050.050.993318
CGTGTAG3150.050.634355
GCAGGGT35950.050.2150159
TATATTC8700.049.9994353
AGCAGGT75650.049.355038
Produced by FastQC (version 0.11.5)