FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11209140
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA26800.073.656711
GAGCAAA123600.059.961811
TAGTAAC72700.057.676812
GCAAAAG127100.056.8624153
CGAAAGC46150.052.04873
AGCAAAA149050.049.2431262
TAACAAG93050.045.50895
TATCGTG23100.041.9593162
CGCACTT12950.039.0443465
ACGCACT18200.038.9713444
GTAGTAA122500.037.0456471
ATATCGT26200.036.4651831
TAGCCGT27250.036.07849
ATCGTGT27200.035.375873
TCGTGTA27500.034.9851154
CACGCAC17400.034.7143
CAAAAGC227750.032.684584
ATAAGTG58800.031.2902988
GTAACAA138600.031.059374
AAAGCAG298300.030.887026
Produced by FastQC (version 0.11.5)