FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n01_SMART_P1_Demulti.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n01_SMART_P1_Demulti.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGG13.7037037037037033No Hit
ATTAAAAACACCCTTGTTTCTACTAATAACCCGGCGGCCCAAAATGCCGA13.7037037037037033No Hit
GTGTCACATATTTGTTGAGGGCAAAAGACTATGGACTGGAAGGCAGAAAG13.7037037037037033No Hit
CCAGAGAAACAAACTAGAGGCATATTTGGCGCAATCGCGGGTTTCATAGA13.7037037037037033No Hit
CTATATGTATATCTGTAGTCTTTGATGAACAATTGGAGAGCCATCTGGGC13.7037037037037033No Hit
TCATATTCCATACTTTTGGCTGGACCATGGGCTGGCATCACTACAGCATT13.7037037037037033No Hit
TTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCG13.7037037037037033No Hit
GATGCAGAGAGAGGTAAATTAAAAAGAAGGGCTATTGCAACACCCGGGAT13.7037037037037033No Hit
AAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGC13.7037037037037033No Hit
ATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCT13.7037037037037033No Hit
ATCAGGAAGAATCACAGTCTCTACCAAAAGAAGCCAACAAACTGTAATCC13.7037037037037033No Hit
GTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAA13.7037037037037033No Hit
TTATCACTGTTACTCCAATGCTCATATCAGCTGACTCGTTTATTCCAGAC13.7037037037037033No Hit
TCCCTGAAGTCTGCTTAAAGTGGGAGCTAATGGATGAGAATTATCAGGGA13.7037037037037033No Hit
TCTCTGCATCTTTGGTCATCGTGTTCAATGTCAAAGCTCTTATTAGATAG13.7037037037037033No Hit
CACTTAGTGTTGTCCCCAGTGATTGTGAAAGAAAGCTCAGTGTCTTGTGA13.7037037037037033No Hit
ATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTA13.7037037037037033No Hit
GAATTACATATCTTTCTTAATTTTGTCCCATTCGCCTTTAACTTTATCTT13.7037037037037033No Hit
AGTAAAACCGGGAGACATACTTTTGATTAACAGCACAGGGAATCTAATTG13.7037037037037033No Hit
GTGATCTGCATTAATGAATCAGGGGATAACGAAAAAAAAAACAAATAAAC13.7037037037037033No Hit
AATCTATCCACTCCTGCTTGTATTCCCTCATGATTTGGTGCTTTCACTAT13.7037037037037033No Hit
GTGTCTTGTGAATTAGTCATCATTTTTCTCACAACATTTGCCAGTTTGGC13.7037037037037033No Hit
GTAATTAGCGCTTGGTTTAATGACGGCTTGTTTCTTTTCTGTGGCTGCGT13.7037037037037033No Hit
AAAGAAACTGTTATTAGATCTCCTTTTGCAAGCAGAGCTTGTTCCATTTT13.7037037037037033No Hit
ATAGTAAAACCGGGAGACATACTTTTGATTAACAGCACAGGGAATCTAAT13.7037037037037033No Hit
GTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACT13.7037037037037033No Hit
GTTTTGGAGTGTCTGGAATAAACGAGTCAGCTGATATGAGCATTGGAGTA13.7037037037037033No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)