FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n01_SMART_NEGCONTROL_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n01_SMART_NEGCONTROL_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1819
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT160.8796041781198461No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA60.32985156679494226No Hit
ATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAATG50.2748763056624519No Hit
CTTCTAGACTTATTCATTCTGCATATCTGCTCCTTTGTATAAAGGATAAA40.21990104452996154No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA40.21990104452996154No Hit
CTGCATATCTGCTCCTTTGTATAAAGGATAAACAAAAACTTTAAAGTACT30.16492578339747113No Hit
CTATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGA30.16492578339747113No Hit
ATTCTATACATTGGGTTATTGTCTCCATTTCACATATCAGCACACTGAGG30.16492578339747113No Hit
TAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATACAAA30.16492578339747113No Hit
AGTAGAAACAACAGCCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTG30.16492578339747113No Hit
GAATTAGGGAGTAAGAAGCAACATTTGCCCAAGAAGGCTGTGTGCATGAG30.16492578339747113No Hit
ATCATATCCTGGCAAACTTGAGCCTGAAGAGTTTAGAAATATATTGATTC30.16492578339747113No Hit
GCCAAATGTCCTCCAGATTCACCTACATTGTGGCAAATAGCAGGAGCCCT30.16492578339747113No Hit
TTTAATAACTGTGTGATTTGGACCGGCCACTTGTTTCGTCACCTGTACAT20.10995052226498077No Hit
CCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGT20.10995052226498077No Hit
CTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTT20.10995052226498077No Hit
GTCCTGAATTCTCCCTTCTACTTCTGAGAATTCTTTTTCAATCTGATGGA20.10995052226498077No Hit
GATTTAAATGTGTCTAGATATGGATCTCTTTATATTACTTGAAGTTTACT20.10995052226498077No Hit
AATTAGTCTTCAGCCGAGGAGCAGGGAAGGGAAAATCAGGAATAGCCTAG20.10995052226498077No Hit
CCTCCCCTCACCAGGCCCTGCTCTTATTCTTCTCCTTCAGGTCTGGCTGT20.10995052226498077No Hit
AATTTGATGCATGATATTTGGGCAAATTACTATGCCTCATTGAGCCATGG20.10995052226498077No Hit
GAATAAAGACTCACAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCTCTT20.10995052226498077No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC20.10995052226498077No Hit
GTAGTAACAAGAGCTCTCGAGATTGGGAATAGGTAATAAAGAAAATGAAT20.10995052226498077No Hit
CATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATC20.10995052226498077No Hit
TTTATATACAATGGAGGCTTAATAGTGTTAACAGCTGACTTCTCATCAGA20.10995052226498077No Hit
GCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAGCCCCT20.10995052226498077No Hit
TTATATTACTTGAAGTTTACTGGGCTTCTTGGGTGCATAATGTTTTAATC20.10995052226498077No Hit
GTATGAAATAAACTTACTGAAAGTGGTGGGAATGAAAAGACCCTAAATAA20.10995052226498077No Hit
GTGCTAGCCCTCCCTTTTCCACTAATTTGATGCATGATATTTGGGCAAAT20.10995052226498077No Hit
GATCCAAACAATGGCTACTGCTGGAGCTGTCGTTTTTTCTGGGTGTGTCT20.10995052226498077No Hit
GTAATAAAGAAAATGAATCCTGAGGGCTGATAAATCAACAAAGTGAGTCC20.10995052226498077No Hit
GGCAAATGTTGTGAGAAAAATGATGACTAATTCACAAGACACAGAGCTTT20.10995052226498077No Hit
CTTATACACATCTCCGAGCACACGAGACCGAGGCTGATCTCGTATGCCGT20.10995052226498077No Hit
GTTCTGCACACTAGATCTTCTAGACTTATTCATTCTGCATATCTGCTCCT20.10995052226498077No Hit
ATTAGATTCCCTGTGCTGTTAATCAAAAGTATGTCTCCCGGTTTTACTAT20.10995052226498077No Hit
GTTAAAACTATGTTCAAGTGATTATGTCAGTCATTGTAGGAAGAAAAATA20.10995052226498077No Hit
GTTAATACATAATACCAACAAACAGTTGGCTTGTGCTCATTTTTCTGACA20.10995052226498077No Hit
GAATCAACGATCGAAATTTTTGGAGAGGTGAGAATGGGCGGAAAACAAGA20.10995052226498077No Hit
GTCAGTGAAGAGAACTGCATTCTGCTTTGCTTCGGTGGAGCAGCTGCAAA20.10995052226498077No Hit
GCCCTAGAGTCAGAAAGAATAGGTACACGTTTCCTTCAACATTTTCAAAG20.10995052226498077No Hit
ATTCTATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACA20.10995052226498077No Hit
ATACTTTCTTGTTTCTTTACATGTCTAAAAATTTTTAATTGACAACTGAA20.10995052226498077No Hit
ATTATGTTCTTTGTTCTGCACACTAGATCTTCTAGACTTATTCATTCTGC20.10995052226498077No Hit
AAAGCAGGGAGTCAAGGATGGCCAAGGTTTGTGGCCTGAGAAACTGGAAG20.10995052226498077No Hit
GATAACAAATAGTAGCACTGCCATAACTATTTTAATGCATGTGTCAGGAA20.10995052226498077No Hit
AAATGGGACCTTTTTATTGAACGAAGCAAAGCCTACAGCAACTGTTACCC20.10995052226498077No Hit
GAATAGGTACACGTTTCCTTCAACATTTTCAAAGAATCAGCTCATGCACA20.10995052226498077No Hit
GTAGAAACAACAGCAAACCCTAAGGAAGAGGAAGAATATGATTTTCAGAC20.10995052226498077No Hit
AATGTATTCTATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAA20.10995052226498077No Hit
GTAGTAACAAGAGCCGTTCAGATCCAAGCCACTGTTAAAAATCCAAGTAG20.10995052226498077No Hit
GTAGTAACAAGAGCGGCTAAAAATCTACTTATTTAACAAAAATCCCAAAT20.10995052226498077No Hit
TATTCATTCTGCATATCTGCTCCTTTGTATAAAGGATAAACAAAAACTTT20.10995052226498077No Hit
CCTATAGTCCCAGCTACTCAGGAGGCTGAGGCAGGAGGATGGCGTGAACC20.10995052226498077No Hit
TCCCAAGCCTCAGGGCTGATTCCGTGCAGTGGGCAGGCTGTAGCCTCTCT20.10995052226498077No Hit
ACACAATACCTTCCCACAATAGCCACTTCATAATCAGAACAAACATGTGA20.10995052226498077No Hit
GGTTTGGCCTGCGGAGGCCCTCTGTTGATTAGTGTTTCCTCCACTCCTGG20.10995052226498077No Hit
CCAAAAGATACTGATCAATTTGATAACCCTTGGAAGAATCAATGTAAGCT20.10995052226498077No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCG20.10995052226498077No Hit
GTCCAAAATGCCCTCAATGGGAATGGAGACCCAAATAACATGGACAAAGC20.10995052226498077No Hit
CCGTACCAACCATCCACCATTCCCTCCCAACCATTTTCTATGAAACCCGC20.10995052226498077No Hit
ACAACAGCCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGAT20.10995052226498077No Hit
TAGGTACACGTTTCCTTCAACATTTTCAAAGAATCAGCTCATGCACACAG20.10995052226498077No Hit
CAAATAATTTTATTAATTATGTTCTTTGTTCTGCACACTAGATCTTCTAG20.10995052226498077No Hit
GAGATGAGACGTTGCCTCCTTCAGTCACTCCAGCAGATCGAGAGCATGAT20.10995052226498077No Hit
GTGTGGATGGCATGCCATTCTGCTGCATTTGAAGATTTAAGATTGTTAAG20.10995052226498077No Hit
ATACAGAGATGAAGCATTAAACAACCGGTTCCAGATCAAAGGTGTTGAGC20.10995052226498077No Hit
GTATGTTAACTTCTGGTAGTTGTTGTTATGGGTTATACTTTCTTGTTTCT20.10995052226498077No Hit
AACACGAGCGGCTAAAAATCTACTTATTTAACAAAAATCCCAAATACAAT20.10995052226498077No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGAGC100.00651747145.08
TAGTAAC100.00651747145.02
AGTAACA100.00651747145.03
TAACAAG100.00651747145.05
GTAACAA100.00651747145.04
GTAGTAA100.00651747145.01
Produced by FastQC (version 0.11.5)