FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n01_FluB_99.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n01_FluB_99.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2936876
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA119470.4067927961548258No Hit
ACAACAGCGAGGCTAGTTGAAATTTATGGGTGAGGAATATGTTGCACCAG68780.23419442972737017No Hit
GTAGTAACAAGAGCGAGGCTAGTTGAAATTTATGGGTGAGGAATATGTTG65770.22394544407050213No Hit
GTAGTAACAAGAGCGAAACCCCATCTCAAAAAAACAAAACCCAAAACACC62500.21281116397151256No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA51970.17695673906559214No Hit
GTAGTAACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACA43760.14900186456629425No Hit
GTTTAGGTCTCTCTCTCCTTGACACCTGACACAATACCTTCCCACAATAG42740.14552878637027916No Hit
CTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTT40330.13732278788753766No Hit
GCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAGCCCCT34660.11801655909204202No Hit
GCCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACG34600.11781226037462936No Hit
TCTTTGTTCTGCACACTAGATCTTCTAGACTTATTCATTCTGCATATCTG30870.1051116901088095No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA115600.0100.357731
TAGTAAC128250.090.1577762
AAGAGCG32800.089.722299
GTAACAA130000.088.498014
AGTAACA136200.085.108173
TAACAAG137950.083.29285
AGCACGC21550.083.107131
GCACGCA23550.075.7258452
AACAGCG16000.074.759063
CAGCGAG15000.072.976935
CACGTCG300.001937706672.4859
ACAGCGA16900.069.91994
ACAAGAG166600.068.9608157
CACGCAC26050.068.458483
AACAAGA169900.067.5441066
CAAGAGC164050.063.670158
ACGTGCA13200.057.6653942
ACAACGC19500.056.501138
AAACACG40100.056.042465
CGCACTT33050.051.1074685
Produced by FastQC (version 0.11.5)