FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n01_FluB_95.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n01_FluB_95.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2995696
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA102720.3428919356303176No Hit
GTAGTAACAAGAGCGGCTAAAAATCTACTTATTTAACAAAAATCCCAAAT42360.14140286597839033No Hit
GTAGTAACAAGAGCGAAACCCCATCTCAAAAAAACAAAACCCAAAACACC41920.13993409211081498No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA41910.1399007108865519No Hit
CTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTT34880.11643371022960942No Hit
GTAGTAACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACA31750.1059853870352666No Hit
GTAGTAACAAGAGCCAAAATAAAACTCTTTTCCTTATAAATTACACAATC31280.10441646949490202No Hit
GTAGTAACAAGAGCTCTCGAGATTGGGAATAGGTAATAAAGAAAATGAAT30420.10154568420827748No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA113050.0113.470891
TAGTAAC120650.0106.292932
GTAACAA131850.096.988974
AGTAACA136150.094.138763
TAACAAG140250.091.076625
AGCACGC37800.077.5026861
CAAGAGC156350.073.621698
GCACGCA39950.073.310892
ACAAGAG174650.073.004637
TACGGCG406.67181E-572.48584145
AAGAGCG32250.071.699179
AACAAGA188200.067.9104466
CGGTGCA7200.065.446252
CGCACTT44000.061.1258855
CACGCAC47900.060.8407363
ACGCACT51900.059.923044
AAACACG56200.058.1760335
ACGTGCA20800.056.810442
AAGAGCC77900.056.2021189
ACAACGC18600.053.3901068
Produced by FastQC (version 0.11.5)