FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n01_FluB_85.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n01_FluB_85.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2741782
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCGGCAGCGATAGTTCTGTGACATTATGTACTGAGTA81260.2963765901154796No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA69980.2552354636510124No Hit
GTAGTAACAAGAGCGAAACCCCATCTCAAAAAAACAAAACCCAAAACACC55400.20205836933789778No Hit
GTAGTAACAAGAGCGAAACTCCATCAAAAAGAAAAAAAAAGAAAGAGAGA51610.18823524262687552No Hit
GTAGTAACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACA44730.16314207329393804No Hit
GTAGTAACAAGAGCGAAACTCCGTCTCAAAAACAAGAAAAAAAGAAAAAG42400.15464395054019614No Hit
GTAGTAACAAGAGCCAAAATAAAACTCTTTTCCTTATAAATTACACAATC38810.14155027642606158No Hit
CAACAGCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAG31860.1162017986842134No Hit
GTTTAGGTCTCTCTCTCCTTGACACCTGACACAATACCTTCCCACAATAG31840.11612885342452463No Hit
GTAGTAACAAGAGCTCTCGAGATTGGGAATAGGTAATAAAGAAAATGAAT31590.11521703767841497No Hit
ACAACAGCCGGCAGCGATAGTTCTGTGACATTATGTACTGAGTAAACACT30420.11094973998662183No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA29320.10693775070373938No Hit
GTAGTAACAAGAGCTGTTATCTCTTTATAAAAATTCAAGCAACCCTGAAA28090.10245161723287993No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA113450.0119.008751
TAGTAAC126800.0106.230932
GTAACAA136050.098.5820164
TAACAAG142500.094.0181355
AGTAACA144250.093.5811543
AGCACGC29600.092.108531
GCACGCA30650.089.409952
AAGAGCG34950.088.978929
ACAAGAG162000.082.5578467
CACGCAC33400.082.26543
CAAGAGC166350.079.483878
AACAAGA177550.075.3355946
AAACACG55800.066.7810755
ACGCACT49000.066.1357044
CGCACTG5900.063.8963245
CGCACTT41900.063.1543665
AAGAGCC93500.060.0851449
AAGAGCT45050.059.3756759
TCCGATT11600.058.741758
AACAACG23250.058.615427
Produced by FastQC (version 0.11.5)