FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n01_FluB_76.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n01_FluB_76.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3287675
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAATG1001253.045465260404389No Hit
GTAGTAACAAGAGCCGTTCAGATCCAAGCCACTGTTAAAAATCCAAGTAG331031.0068817629479798No Hit
GTTCAGATCCAAGCCACTGTTAAAAATCCAAGTAGTACCTAGCGCAAGTT244140.7425916491137354No Hit
ATTCTATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACA244090.742439565954664No Hit
AACAAGAGCCGTTCAGATCCAAGCCACTGTTAAAAATCCAAGTAGTACCT241620.7349266578965379No Hit
CTATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAA215730.6561779981293772No Hit
CATATAAATATATCCTTTTATCATATCCTGGCAAACTTGAGCCTGAAGAG169020.5141019109248938No Hit
TCTTCACACAGCCCCCAACTCCAAACACGCCTCTCAGAAGGACTTTTGTA149170.4537248967735558No Hit
GAATTAGGGAGTAAGAAGCAACATTTGCCCAAGAAGGCTGTGTGCATGAG146980.44706365440622936No Hit
GTAGTAACAAGAGCGGCTAAAAATCTACTTATTTAACAAAAATCCCAAAT126440.38458789265970633No Hit
GATCCAAGCCACTGTTAAAAATCCAAGTAGTACCTAGCGCAAGTTCAAGA107980.32843879033055273No Hit
GTTCAAGACACTGTGAAAGGAGGGGAGAGTGTTCACACTCAAGTGAGGAA106800.3248496277764682No Hit
GTTAAAAATCCAAGTAGTACCTAGCGCAAGTTCAAGACACTGTGAAAGGA103110.3136258906370003No Hit
AACAACAGCTGTCTGCCACTTCTCTCTTCTTCACACAGCCCCCAACTCCA102820.3127438083143863No Hit
ATATGAAAGTCAGCTCTAGAAATAACAGAATGGAATTAGGGAGTAAGAAG102620.3121354756781008No Hit
ATGCAGAGGAGTTCTAATTGGAAAGAAGAAATGAGGAAGCTGCCCTAGAG98610.2999384063205761No Hit
TGTATACACACACATATAAATATATCCTTTTATCATATCCTGGCAAACTT89770.2730501037967561No Hit
GCCCTAGAGTCAGAAAGAATAGGTACACGTTTCCTTCAACATTTTCAAAG88180.2682138593382862No Hit
CCCTAAGGAAGAGGAAGAATATGATTTTCAGACTTGTCATGTTATGTTAC84830.2580242876805037No Hit
CTTTATATTCTTTGTCTGTATGCAGAGGAGTTCTAATTGGAAAGAAGAAA84250.2562601230352757No Hit
ATATTAGACATTTCCATATTTTTTGAAAGCAGAACTTGCAGACTCGTCAA83440.2537963758583193No Hit
GTCTAATATCTTCTTTAATTTTGAAGAATAGCTTTACTGGTGCAGAATTC81790.24877763160896377No Hit
GTGAAAGGAGGGGAGAGTGTTCACACTCAAGTGAGGAAAATGGAACTTGA78610.239105142692024No Hit
CTTTATATACAATGGAGGCTTAATAGTGTTAACAGCTGACTTCTCATCAG78330.23825347700122426No Hit
GTATATAAAGAATCTCTTAATTATTGCTGCTTTCAATATTCTCTCTGTAT77440.2355463967697537No Hit
GTAGTACCTAGCGCAAGTTCAAGACACTGTGAAAGGAGGGGAGAGTGTTC77260.23499889739709673No Hit
GTATGCAGAGGAGTTCTAATTGGAAAGAAGAAATGAGGAAGCTGCCCTAG76970.23411681507448273No Hit
CCCAAATACAATGCAATTTTATTAATGATAGTTCTCATGTTGGACATGAA74410.22633015733002806No Hit
CTGTGAAAGGAGGGGAGAGTGTTCACACTCAAGTGAGGAAAATGGAACTT73070.22225432866691508No Hit
CAACAGCTGTCTGCCACTTCTCTCTTCTTCACACAGCCCCCAACTCCAAA70190.21349433870440354No Hit
ATCATATCCTGGCAAACTTGAGCCTGAAGAGTTTAGAAATATATTGATTC70040.21303808922718942No Hit
ACTTTATATTCTTTGTCTGTATGCAGAGGAGTTCTAATTGGAAAGAAGAA69480.21133475784558997No Hit
AGAAATAACAGAATGGAATTAGGGAGTAAGAAGCAACATTTGCCCAAGAA67650.20576851422357745No Hit
GTATTCTATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAA66660.20275726767396413No Hit
ATGTATATGTATACACACACATATAAATATATCCTTTTATCATATCCTGG66110.20108435292417892No Hit
TTCTTTGTCTGTATGCAGAGGAGTTCTAATTGGAAAGAAGAAATGAGGAA65260.19849893921996548No Hit
AAATAATGTATTCTATATATATCTTCACACAAATATGAAAGTCAGCTCTA65250.19846852258815123No Hit
AAGTAGTACCTAGCGCAAGTTCAAGACACTGTGAAAGGAGGGGAGAGTGT63000.19162478042993908No Hit
TCTCTCTTCTTCACACAGCCCCCAACTCCAAACACGCCTCTCAGAAGGAC60700.1846289551126556No Hit
GAATAGGTACACGTTTCCTTCAACATTTTCAAAGAATCAGCTCATGCACA58580.17818062916802907No Hit
GAGCCGTTCAGATCCAAGCCACTGTTAAAAATCCAAGTAGTACCTAGCGC57770.17571688199107272No Hit
AATGTATTCTATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAA56900.1730706350232307No Hit
GTATATGTATACACACACATATAAATATATCCTTTTATCATATCCTGGCA55510.16884272320104632No Hit
GTGTACACTGTACCCAATGTGTAGTCTAGACAAAGATTTTAAATCAACTA55120.16765647456028956No Hit
TCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAATGGAATTAG54700.16637897602408996No Hit
ATTTATATGTGTGTGTATACATATACATTTTAAATATATAAGTAATATAA54580.16601397644231866No Hit
CCCTATCTCTGTATATTTAAATTTTTAAGAAGATTCCACATGTAAGTGAG54370.16537522717421888No Hit
CTCTAGGGCAGCTTCCTCATTTCTTCTTTCCAATTAGAACTCCTCTGCAT53070.16142106503836298No Hit
ATTTTAAATATGTTTGAAGAATTAAAGAAACCATGTCTTTAAATGTCTTA52040.15828815196149254No Hit
GTATACACACACATATAAATATATCCTTTTATCATATCCTGGCAAACTTG50420.15336065760757983No Hit
CTTCTTCACACAGCCCCCAACTCCAAACACGCCTCTCAGAAGGACTTTTG49780.15141399317146617No Hit
GAATGGAATTAGGGAGTAAGAAGCAACATTTGCCCAAGAAGGCTGTGTGC49480.15050149421703787No Hit
ATATAAAGAGATCCATATCTAGACACATTTAAATCAAAGTGTTGAAATAC49430.1503494110579665No Hit
ATCCAAGCCACTGTTAAAAATCCAAGTAGTACCTAGCGCAAGTTCAAGAC49130.1494369121035382No Hit
GTGTACACTGCTCAGGTGATGGGTAACCAAAATCTCAGAAAACACCACTT48960.14891982936269552No Hit
GTCTGCCACTTCTCTCTTCTTCACACAGCCCCCAACTCCAAACACGCCTC48150.14645608218573916No Hit
GTAGAAACAACAGCTGTCTGCCACTTCTCTCTTCTTCACACAGCCCCCAA47920.14575649965401083No Hit
AAACAACAGCTGTCTGCCACTTCTCTCTTCTTCACACAGCCCCCAACTCC47220.14362733542701148No Hit
CTTTATATTACTTGAAGTTTACTGGGCTTCTTGGGTGCATAATGTTTTAA46160.14040317245469824No Hit
GTCTGTATGCAGAGGAGTTCTAATTGGAAAGAAGAAATGAGGAAGCTGCC45520.13845650801858456No Hit
AATATATCCTTTTATCATATCCTGGCAAACTTGAGCCTGAAGAGTTTAGA45330.13787859201411332No Hit
TATTAGTAGTAACAAGAGCCGTTCAGATCCAAGCCACTGTTAAAAATCCA45060.13705734295512786No Hit
ATTAGTAGTAACAAGAGCCGTTCAGATCCAAGCCACTGTTAAAAATCCAA45030.13696609305968505No Hit
GCCCCCAACTCCAAACACGCCTCTCAGAAGGACTTTTGTAGCTTGCTTAA43080.1310348498559012No Hit
AGTAGAAACAACAGCTGTCTGCCACTTCTCTCTTCTTCACACAGCCCCCA42730.12997026774240156No Hit
TTTTAATGCTGAATGATATTCCTGTGTGTGTGTGTGTCTTTGTGTTGACA42460.1291490186834161No Hit
CTATTAAGCCTCCATTGTATATAAAGAATCTCTTAATTATTGCTGCTTTC40750.12394777464317487No Hit
TCTGTATGCAGAGGAGTTCTAATTGGAAAGAAGAAATGAGGAAGCTGCCC40660.1236740249568464No Hit
ACCTAGCGCAAGTTCAAGACACTGTGAAAGGAGGGGAGAGTGTTCACACT40460.12306569232056089No Hit
GTTCTAATTGGAAAGAAGAAATGAGGAAGCTGCCCTAGAGTCAGAAAGAA40440.12300485905693233No Hit
CACTAGACAAATACTTTACTTTTATTTTTTTTATTTCAGTAGGTTTTTGG40310.12260944284334674No Hit
TATCTAGACACATTTAAATCAAAGTGTTGAAATACAGAGAGAATATTGAA40220.12233569315701827No Hit
CAAATATGAAAGTCAGCTCTAGAAATAACAGAATGGAATTAGGGAGTAAG40110.12200111020706121No Hit
TATGAAAGTCAGCTCTAGAAATAACAGAATGGAATTAGGGAGTAAGAAGC39460.12002402913913326No Hit
GTAAATAATTGTTTTACTCCCTATCTCTGTATATTTAAATTTTTAAGAAG39220.11929402997559065No Hit
GGCTTAATAGTGTTAACAGCTGACTTCTCATCAGAAACCATGAAATTTAG38780.11795569817576251No Hit
TTTATATACAATGGAGGCTTAATAGTGTTAACAGCTGACTTCTCATCAGA38770.11792528154394823No Hit
GCTCTAGAAATAACAGAATGGAATTAGGGAGTAAGAAGCAACATTTGCCC38470.11701278258951994No Hit
AGTGTACACTGCTCAGGTGATGGGTAACCAAAATCTCAGAAAACACCACT38310.11652611648049152No Hit
GTTCACACTCAAGTGAGGAAAATGGAACTTGAAGAACTTTATATTCTTTG37230.11324112024454973No Hit
ATACAATGGAGGCTTAATAGTGTTAACAGCTGACTTCTCATCAGAAACCA37120.11290653729459268No Hit
CTTTAAATGTCTTACAAAATAGAAACTTTCAATAAAGAGATAGAAACTGC36430.11080778969940763No Hit
ACCTATATCTTCTCATTTCCTCCCCTCTTTGACCAGTAAATAATTGTTTT36280.1103515402221935No Hit
AAATAACAGAATGGAATTAGGGAGTAAGAAGCAACATTTGCCCAAGAAGG36150.10995612400860791No Hit
TATAAATATATCCTTTTATCATATCCTGGCAAACTTGAGCCTGAAGAGTT35550.10813112609975135No Hit
ATGTAGGTGAATCTGGAGGACATTTGGCTAAGTGAAATTAGCCAAACACA35040.10657987787722327No Hit
GTATAACCCATAACAACAACTACCAGAAGTTAACATACAGTGTCTGAGGA35030.106549461245409No Hit
GTCTAGATATGGATCTCTTTATATTACTTGAAGTTTACTGGGCTTCTTGG34950.10630612819089479No Hit
GAATATAAAGTTCTTCAAGTTCCATTTTCCTCACTTGAGTGTGAACACTC34870.10606279513638056No Hit
TACCTATTCTTTCTGACTCTAGGGCAGCTTCCTCATTTCTTCTTTCCAAT34830.10594112860912346No Hit
GTTAACATACAGTGTCTGAGGAAGCCCAGATATTGGACACACTAGACAAA33670.10241279931866745No Hit
ATGTATCATAGGAGCCTCAAATGGAGAACAGAGAAATAACTGGGAAGAAT33440.10171321678693909No Hit
ATATACAATGGAGGCTTAATAGTGTTAACAGCTGACTTCTCATCAGAAAC33420.10165238352331055No Hit
GTCTGAGGAAGCCCAGATATTGGACACACTAGACAAATACTTTACTTTTA33350.10143946710061062No Hit
ATGTAAGTGAGATCATGCGATATGTTTCTTTCTGTGTTTGGCTAATTTCA32960.10025321845985385No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACGA100.00709357144.98654
GTAGTAA92950.0101.6432341
TAGTAAC94450.0100.085962
AGTAACA114450.082.722743
GTAACAA115350.082.0144654
TAACAAG116000.081.2424245
CGAGCCG1800.076.5206455
AAGAGCG32100.075.646899
ATCTTCA222650.066.837097
CGTGCCG451.1948139E-464.438445
ACAAGAG147700.063.4555057
CCGTTCG350.003570051262.1304559
CAAGAGC150350.061.903178
TGACTCT210700.058.621426145
AAGAGCC103850.053.8144849
CCCTAAG22850.051.0884931
CCTAAGG23200.050.620282
CTTCACA321300.048.6846479
GAGCGAA604.944192E-448.338391
CGGTGCA1800.048.328832
Produced by FastQC (version 0.11.5)