FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n01_FluB_75.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n01_FluB_75.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2874985
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA89640.31179293109355355No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA64710.2250794351970532No Hit
GTAGTAACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACA49760.17307916389128986No Hit
GTAGTAACAAGAGCGAAACTCCATCTCTAAAAAAAGAAAAAAAGAGTTGT47900.16660956491946916No Hit
CAACAGCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAG47880.16653999933912697No Hit
GTATAAAGGATAAACAAAAACTTTAAAGTACTTCTCTCTTCTGTCCTTCC41960.14594858755784812No Hit
CTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTT41460.14420944804929417No Hit
GTTTAGGTCTCTCTCTCCTTGACACCTGACACAATACCTTCCCACAATAG39370.13693984490353864No Hit
TCTTTGTTCTGCACACTAGATCTTCTAGACTTATTCATTCTGCATATCTG37840.13161807800736353No Hit
GCCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACG35170.12233107303168539No Hit
CTTCTAGACTTATTCATTCTGCATATCTGCTCCTTTGTATAAAGGATAAA34410.11968758097868337No Hit
GCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAGCCCCT33510.11655712986328624No Hit
ATTATGTTCTTTGTTCTGCACACTAGATCTTCTAGACTTATTCATTCTGC31520.10963535461924148No Hit
CTATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGA29930.10410489098203991No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAAC77200.0101.1361542
GTAGTAA78950.099.7416151
GTAACAA88950.087.857914
TAACAAG91450.085.297565
AGTAACA96300.081.227523
AAGAGCG22750.080.290989
TTACGGA755.638867E-1177.3283
GTTACGG851.6916601E-1068.230582
CAAGAGC113750.066.590548
ACAAGAG117200.066.547587
GGTTACG1051.6370905E-1162.151541
CGGTGCA3250.060.2266162
AACAAGA132800.059.120546
AAACACG36500.056.6056825
AGCACGC10750.053.286521
GCACGCA11050.052.4850652
AAGCGGT3000.045.91352
GAAGCGG10150.045.006291
ACGAGCT8150.044.475464
ACGTGCA13300.044.151092
Produced by FastQC (version 0.11.5)