FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n01_FluB_68.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n01_FluB_68.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3259415
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA75000.23010264111811476No Hit
GTAGTAACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACA59640.18297762021712485No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA43200.1325391212840341No Hit
ATATTAGACATTTCCATATTTTTTGAAAGCAGAACTTGCAGACTCGTCAA39140.12008289831150683No Hit
GCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAGCCCCT39110.11999085725505956No Hit
GTTTAGGTCTCTCTCTCCTTGACACCTGACACAATACCTTCCCACAATAG38520.11818071647826374No Hit
CCCTAAGGAAGAGGAAGAATATGATTTTCAGACTTGTCATGTTATGTTAC34900.10707442900029607No Hit
GTCTAATATCTTCTTTAATTTTGAAGAATAGCTTTACTGGTGCAGAATTC33350.10231897441718837No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA113200.077.632331
TAGTAAC123200.071.020392
GTAACAA132400.065.8116764
TAACAAG135100.064.2281345
AGTAACA146150.060.1160283
CGAGCTA4050.059.0670665
GCACGCA24400.056.1511272
ACAAGAG171050.050.6821027
CAAGAGC157750.050.314288
CGTCCAG9400.050.127035
AGCACGC31550.048.9515761
CACGCAC28300.048.6691363
AAGAGCG20700.045.5218129
AAGAGCC92450.044.29849
ACGCACT36150.041.309074
AACAAGA215150.040.4994966
GTCGTCC11100.040.4906923
CGTGCGA750.001484743838.662083
CGAAAGC8400.037.9716874
CGTGCAA3250.037.918583
Produced by FastQC (version 0.11.5)