FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n01_FluB_55.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n01_FluB_55.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2652115
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCGAAACCCCATCTCAAAAAAACAAAACCCAAAACACC242780.9154203343369348No Hit
TCATTACATTGTGTCGTCACAAGTCAATTTTGAGTTTTTATATAAATAAG90790.34233055504757526No Hit
GTACTACAGATTGCTTCTCCTAATTTACCAACATCAATTAATTTATCTAA88420.3333942909715453No Hit
ATTATATGTACTACAGATTGCTTCTCCTAATTTACCAACATCAATTAATT86160.32487279020706117No Hit
CTTATACATAATGGATATGAAAGCACAGAAGAGGTGGAGCCAGGACAACT79950.3014575159825271No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT73470.27702418635692644No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCCTT72040.27163226330683243No Hit
GTTAAAACTATGTTCAAGTGATTATGTCAGTCATTGTAGGAAGAAAAATA69720.2628845280087779No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT66150.2494235732613405No Hit
TTATAGATATTTCTTCACAAGAGCTGAATTTCCCATGGAGCTCTGCTTTA59270.22348201341193724No Hit
ATGTAATGATGGCATTGAGTCTTTGAGAACAGTTTTTTACTCCTTTATAC57340.21620480258209016No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT55560.20949317808616896No Hit
TTCTAGCTCTGATAAAGTTACAAGATCAATTATATGTACTACAGATTGCT54600.20587342554904292No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA53860.20308319963500832No Hit
AGTTAAAACTATGTTCAAGTGATTATGTCAGTCATTGTAGGAAGAAAAAT53220.20067003127692426No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC52940.19961427012026253No Hit
ATGTACTACAGATTGCTTCTCCTAATTTACCAACATCAATTAATTTATCT52840.19923721256431187No Hit
GTATAAAGGAGTAAAAAACTGTTCTCAAAGACTCAATGCCATCATTACAT52550.19814374565205506No Hit
ATATATTTCTTAATCTGTAATGATAATGTGATTTCCAAGCATTTCATCAT52480.19787980536288963No Hit
TATTTATATAGTTAAAACTATGTTCAAGTGATTATGTCAGTCATTGTAGG48540.18302373765843488No Hit
TATATATTCTAGCTCTGATAAAGTTACAAGATCAATTATATGTACTACAG48530.1829860319028398No Hit
ACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC46900.17683999374084458No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC46310.17461535416073587No Hit
GTACTAAATATATTTCTTAATCTGTAATGATAATGTGATTTCCAAGCATT45860.17291859515895805No Hit
ATACTAAACACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGC41050.1547821267177328No Hit
GGATATGAAAGCACAGAAGAGGTGGAGCCAGGACAACTTAAAATCCAAGG40480.15263289864881424No Hit
TTCTTAATCTGTAATGATAATGTGATTTCCAAGCATTTCATCATTTGGAA39540.14908855762287834No Hit
GTAGAAACACGAGCGAAACCCCATCTCAAAAAAACAAAACCCAAAACACC38650.14573274537491776No Hit
GATTATGTCAGTCATTGTAGGAAGAAAAATAAGTTTATTGCCGCATTATT37750.14233922737136212No Hit
GTTCAAGTGATTATGTCAGTCATTGTAGGAAGAAAAATAAGTTTATTGCC37180.14018999930244352No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT37170.14015229354684847No Hit
TATCTATAATGCTCGAACCATTTCAGATTCTTACAATTTGTTCTTTTATC36720.13845553454507062No Hit
CAATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCC36420.13732436187721875No Hit
CTTCTTATACATAATGGATATGAAAGCACAGAAGAGGTGGAGCCAGGACA35920.1354390740974656No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA35600.1342324899184236No Hit
TTTATATAGTTAAAACTATGTTCAAGTGATTATGTCAGTCATTGTAGGAA35320.13317672876176184No Hit
GTAATGATAATGTGATTTCCAAGCATTTCATCATTTGGAAGAGTTGGAGA35060.1321963791162902No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG34810.13125373522641365No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT34770.1311029122040334No Hit
ATCATGTAGTTGTACTAAATATATTTCTTAATCTGTAATGATAATGTGAT34570.13034879709213212No Hit
ATGTTCAAGTGATTATGTCAGTCATTGTAGGAAGAAAAATAAGTTTATTG34310.12936844744666048No Hit
ATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG33860.12767168844488266No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA32340.12194041359443313No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGAAAGCTGAA32060.12088465243777137No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA32020.12073382941539111No Hit
CTGTAATGATAATGTGATTTCCAAGCATTTCATCATTTGGAAGAGTTGGA31340.11816983803492685No Hit
GTAGAAACAACAGCCAGCGGAAGACAAGTTTAGTTCCAATGACAAAACAA30100.11349432434113904No Hit
CAATTATATGTACTACAGATTGCTTCTCCTAATTTACCAACATCAATTAA29950.11292873800721312No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA29860.11258938620685753No Hit
CCTTTATTGTTTCTTTGGCCTGGATTTCTTTTTGGTAACTGTGTCTCAAA29470.1111188617386501No Hit
GATCTATACTAAACACTGATCAGAGGAACATGATTCTTGAGGAACAATGC29160.10994998331520314No Hit
GTAGAAACAACAGCAACAAAAAAGAAAGGCCTGATTCTGGCTGAGAGAAA29030.10945980849246734No Hit
ACAACAGCCAGCGGAAGACAAGTTTAGTTCCAATGACAAAACAAAGTTCC28920.10904504518092163No Hit
TCTTAAAATTCAGTAGGTAGTGGCGATTTTGCATGTGAAAACAAGGGTGG28690.1081778128022352No Hit
GTTTATTGTCTCTTTGTTTGGACCTTTTATTCGTATTTTCATGTTTATTC27990.10553840991058079No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT27270.10282359550773629No Hit
AACACGAGCGAAACCCCATCTCAAAAAAACAAAACCCAAAACACCATCAT27260.10278588975214122No Hit
ATATATTCTAGCTCTGATAAAGTTACAAGATCAATTATATGTACTACAGA26970.10169242283988439No Hit
GTAATATTCAGTGAATCAAAGGTGGGGAGAGAAAATTCTCCTGCATCAAA26880.10135307103952883No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT26680.10059895592762758No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC26680.10059895592762758No Hit
TTCTTAATCTGTGGGCCATAGCCTCAAGCATGCTATGTTGACCCACTGGC26660.10052354441643745No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA80400.0106.301434
TAACAAG84200.0101.3317955
AGCACGC1800.0100.6983951
TAGTAAC88650.097.062932
CACGCAC1950.089.219133
AGTAACA97300.088.433993
GCACGCA2000.086.9886552
AAGAGCG41000.084.862189
AACAAGA110500.077.213916
AAACACG32000.076.3416065
AGCGGCG801.0004442E-1072.486447
AACACGT9750.071.3753056
GTAGTAA130650.067.0921861
CGGTGCA2850.063.5881962
ACAAGAG137550.062.394737
ACGAGCG10350.062.331349
ACGTGCG4150.061.1331449
GTAGAAA62000.060.5749631
AACAACG16050.059.6150177
TAGAAAC64300.058.1728132
Produced by FastQC (version 0.11.5)