FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n01_FluB_53.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n01_FluB_53.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2399528
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAATG189290.7888634764837085No Hit
GTAGTAACAAGAGCCGTTCAGATCCAAGCCACTGTTAAAAATCCAAGTAG96780.4033293214332152No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA76710.3196878719481498No Hit
GTGTTTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTTAGTA44260.1844529424120077No Hit
ATTCTATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACA42850.17857678676806438No Hit
CTATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAA39480.16453235803041266No Hit
GTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTTAGTA36620.15261334729163403No Hit
AACAAGAGCCGTTCAGATCCAAGCCACTGTTAAAAATCCAAGTAGTACCT36330.15140477627266696No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA35670.1486542353329488No Hit
GTTCAGATCCAAGCCACTGTTAAAAATCCAAGTAGTACCTAGCGCAAGTT35330.14723729000036673No Hit
CATATAAATATATCCTTTTATCATATCCTGGCAAACTTGAGCCTGAAGAG32660.13611010165332516No Hit
GTAGTAACAAGAGCGAAACCCCATCTCAAAAAAACAAAACCCAAAACACC29100.12127385052393637No Hit
TCTTTGTTCTGCACACTAGATCTTCTAGACTTATTCATTCTGCATATCTG27930.11639789158534512No Hit
GAATTAGGGAGTAAGAAGCAACATTTGCCCAAGAAGGCTGTGTGCATGAG25720.10718774692356162No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA96500.0108.2706451
TAGTAAC98100.0106.043622
GTAACAA101200.0102.72364
AGTAACA103850.0100.032543
AGCACGC12450.088.521391
TAACAAG117700.088.2615055
GATAGCG2200.082.3931961
AACAAGA133000.078.053616
CAAGAGC133450.074.3572168
ACGTGCA13050.073.327192
CGTGCAC7200.070.48023
GCACGCA16050.068.6546862
AAGAGCG14700.068.538689
CACGCAC16300.068.491193
CGCACTT16550.062.6382565
AAGAGCC78650.057.783939
ACGCACT23100.057.744084
ATCTTCA49350.057.1348047
AACACGT14500.056.9952246
TAGAAAC56000.056.1827932
Produced by FastQC (version 0.11.5)