FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n01_FluB_50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n01_FluB_50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3069469
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAACAGCCAGAGTCAAAACACCTTCTTGTATTTGCCAAACATTCATTAA85890.27982038587130215No Hit
GTAGTAACAAGAGCCGGGTTATTAGTAGAAACACGAGCCCGGGTTATTAG31590.10291682372423373No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGAGCG3350.041.11115145
GCCAGAG34950.037.7463537
CCGACTG5200.037.6366739
ACAGCCA48100.035.7193534
AAGAGCG20700.035.717479
AGCCAGA42700.032.7664766
GTAGTAA396300.032.4590641
CGTGCAT13850.032.4520573
CCCTAAG14000.032.1122061
TAGTAAC402350.031.945682
TAACAAG408300.031.1600465
GTAACAA411900.030.9581054
ACGTGCA27100.030.7635172
AGTAACA419800.030.6172833
GCCGACT6550.029.8794988
AAGAGCA57750.028.36669
CAGCCAG51300.028.1213445
ACAAGAG437450.027.7381737
GCAGACC12550.027.7235168
CAAGAGC395300.027.6885978
Produced by FastQC (version 0.11.5)