FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n01_FluB_46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n01_FluB_46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1686410
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACA110910.6576692500637449No Hit
ATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAATG66570.3947438641848661No Hit
ACAACAGCCACTCTTCTTCCTTCCGTTTTTAAGTTATAAAAAGCATTTAA61000.3617151226570051No Hit
GCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAGCCCCT42610.2526669078100818No Hit
GTAGTAACAAGAGCCACTCTTCTTCCTTCCGTTTTTAAGTTATAAAAAGC42550.2523111224435339No Hit
GTTTAGGTCTCTCTCTCCTTGACACCTGACACAATACCTTCCCACAATAG40270.23879127851471468No Hit
AACACGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACAATACC31420.1863129369489033No Hit
CAACAGCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAG31230.18518628328816836No Hit
TCCATATACAGAATCTCAAATGATTCTAAAAATGGAGCCCTTAGGTAATG30240.17931582474012844No Hit
ATGTTTAGGTCTCTCTCTCCTTGACACCTGACACAATACCTTCCCACAAT28750.1704804881375229No Hit
AACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACAATACC28100.16662614666658762No Hit
TTCTAATGCATAGCCAAAGTCAAGAATCACTAGAGCTCTCTTTAAAATTC27130.1608742832407303No Hit
AGTTCACACACTGCCTGAACATTATAATCGCCTAGGGAACAACACACACA24130.14308501491333664No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA23450.13905278075912736No Hit
GTAGTAACAAGAGCCGTTCAGATCCAAGCCACTGTTAAAAATCCAAGTAG22670.134427570994005No Hit
GTAGTAACAAGAGCAGGGGGAGGAGGTAACGATTGGTTCACTGCTTAATT21720.12879430269033035No Hit
AAATATAGTCATAACATTGTGCAGCCGTCCCTACCATCCATCTCCATAAT21340.1265409953688605No Hit
CTTCATAATCAGAACAAACATGTGATTGAGTGGAACAAGCTAGTGCTAGC19560.11598602949460689No Hit
GTATATGGAAGAGTATTTTGGAAACTCTGATGTGCTATTGAAATAGAAGT18340.10875172704146677No Hit
GTAGTAACAAGAGCTCTCGAGATTGGGAATAGGTAATAAAGAAAATGAAT18100.10732858557527529No Hit
TTACTATGCCTCATTGAGCCATGGTTTGCCCATCTTGAGGTGGACATGCC17840.10578684898690116No Hit
CACGTGCACACAGGGGAATCAACTCATACTAGTAGGGGCCTGTCAGAGGG17830.10572755142580986No Hit
CACACACACACACACACATTTTTTAAAGCTTTGTAGGTGATTCTAATGCA17820.10566825386471854No Hit
CTTTAAAATTCATATGTGACATATCCTTTTAACCCCAAAACAACCACACT17570.10418581483743573No Hit
GTAGTAACAAGAGCTAAAAACTCTGTCTCAGAAAAGAAAAAGAAAAAGAA17450.10347424410433999No Hit
CTCCTTGACACCTGACACAATACCTTCCCACAATAGCCACTTCATAATCA17360.10294056605451819No Hit
CACGAGCACACAGGGGAATCAACTCATACTAGTAGGGGCCTGTCAGAGGG17270.10240688800469636No Hit
TTCCTTATCTTTTCATAGCCCCTAGTTCACACACTGCCTGAACATTATAA16980.1006872587330483No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA68500.0108.789411
TAGTAAC69800.0106.549222
TAACAAG71200.0102.825235
GTAACAA74350.098.468834
AGTAACA84050.089.002113
TGCGTAC259.394719E-486.984035
GCGGTGC3700.084.24631
CGAGCTA6500.083.6385045
GCACGCA5250.078.699852
AGCACGC5600.073.7854841
ACAAGAG106850.068.649697
CAAGAGC100150.068.2485358
AACAAGA108350.067.636416
ACGTGCA10650.063.2982442
AAGAGCT43150.062.8236859
TTTCGAG350.00356952162.1314544
GTGCGTA350.00356952162.1314544
CAGCCAC18600.061.5747265
CGTGCAC4600.061.4561123
CGGTGCA2750.060.625242
Produced by FastQC (version 0.11.5)