FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n01_FluB_110.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n01_FluB_110.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1932895
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAATG84320.4362368364551618No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA81060.4193709435846231No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA50480.26116266015484546No Hit
GTAGTAACAAGAGCGGCTAAAAATCTACTTATTTAACAAAAATCCCAAAT42570.22023958880332353No Hit
CTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTT40610.21009935873391983No Hit
GCCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACG36900.19090535181683432No Hit
TCTTTGTTCTGCACACTAGATCTTCTAGACTTATTCATTCTGCATATCTG36770.19023278553672082No Hit
CTTTATAGCAACGCAAAAAATGGATGAACACAGCTGTGTCCTGTGAACTC33820.1749707045649143No Hit
GTAGTAACAAGAGCCGTTCAGATCCAAGCCACTGTTAAAAATCCAAGTAG31750.1642613799507992No Hit
GTATAAAGGATAAACAAAAACTTTAAAGTACTTCTCTCTTCTGTCCTTCC28090.14532605237221888No Hit
CTTCTAGACTTATTCATTCTGCATATCTGCTCCTTTGTATAAAGGATAAA27900.14434307088589915No Hit
ATTATGTTCTTTGTTCTGCACACTAGATCTTCTAGACTTATTCATTCTGC26390.13653095486304223No Hit
GTAGTAACAAGAGCCAAAATAAAACTCTTTTCCTTATAAATTACACAATC25530.13208167024075287No Hit
GTAGAAACACGAGCCAAAATAAAACTCTTTTCCTTATAAATTACACAATC25220.13047785834202064No Hit
AACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGC24440.1264424606613396No Hit
CTATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGA23990.12411434661479283No Hit
CTTAAAATCTACTCTTTTAGCAAAAATCCTGAATACAAATAATTTTATTA23260.12033762827261699No Hit
GTTCAGATCCAAGCCACTGTTAAAAATCCAAGTAGTACCTAGCGCAAGTT23250.12028589240491594No Hit
GTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTTAGTAGG23250.12028589240491594No Hit
GTAGTAACAAGAGCAAACCCTAAGGAAGAGGAAGAATATGATTTTCAGAC22420.1159918153857297No Hit
GTAGAAACAACAGCCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTGG22270.1152157773702141No Hit
AACAAGAGCCGTTCAGATCCAAGCCACTGTTAAAAATCCAAGTAGTACCT21820.11288766332366734No Hit
ATTCTATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACA21820.11288766332366734No Hit
AAATTACACAATCTCAGTTATTTCTTTATAGCAACGCAAAAAATGGATGA19360.10016063986921173No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAAC67750.094.1676942
GTAGTAA70300.090.669921
AGCACGC10800.086.61551
AAGAGCG10250.086.279659
GCACGCA11000.084.36182
GTAACAA75550.083.58194
TAACAAG77100.081.619495
AGTAACA83400.076.584063
ACAAGAG95700.065.5961467
CAAGAGC91650.064.935638
CGACCGG350.003567314762.1415065
CGCACTG2950.061.439345
CACGCAC15800.058.2740483
AACAAGA110250.057.143886
ACGAGCG2700.056.3876574
CGGTGCA2550.051.1753542
GCGGTGC5000.050.7607151
ACGCACT22250.050.5045174
ACAACGC11550.048.953648
CGCACTT16850.048.619125
Produced by FastQC (version 0.11.5)