FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n01_FluB_109.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n01_FluB_109.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3088542
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA127670.4133665658423942No Hit
GTTTAGGTCTCTCTCTCCTTGACACCTGACACAATACCTTCCCACAATAG65710.2127541085729124No Hit
GTAGTAACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACA65080.21071431115393607No Hit
ATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAATG54270.17571397766324692No Hit
GCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAGCCCCT52110.16872038651247093No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA48090.15570453631519338No Hit
ATGTTTAGGTCTCTCTCTCCTTGACACCTGACACAATACCTTCCCACAAT45450.14715681379757828No Hit
CAACAGCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAG44740.14485799448412875No Hit
CTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTT37440.12122224661345062No Hit
GTAGTAACAAGAGCCAAAATAAAACTCTTTTCCTTATAAATTACACAATC37090.12008902582513042No Hit
GCCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACG33490.1084330405738371No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA106050.0116.305811
TAGTAAC113650.0108.943072
GTAACAA129050.095.998674
AGTAACA133450.092.779173
TAACAAG136350.090.752695
GCTATCG259.39652E-486.9815757
ACAAGAG159200.077.4930047
GCACGCA23750.076.9162452
AGCACGC24400.074.889081
CAAGAGC159050.073.9202048
AAACACG45700.071.8560265
AACAAGA179050.069.109926
AAGAGCG23750.066.533279
CACGCAC28300.065.062163
GTAGAAA74950.064.723531
ACGCACT34500.061.7745254
CGCACTT27800.060.495685
GAAACAC55650.058.6176644
ACGTGCA24700.058.4032862
ACGAGCG4800.057.383689
Produced by FastQC (version 0.11.5)