FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n01_FluB_102.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n01_FluB_102.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3265941
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCGGCAGCGATAGTTCTGTGACATTATGTACTGAGTA213980.6551863612967901No Hit
ATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAATG183450.5617064117202362No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA99840.30570056225755454No Hit
GTAGTAACAAGAGCCGTTCAGATCCAAGCCACTGTTAAAAATCCAAGTAG98540.30172008618649265No Hit
AGTAGAAACAACGCACTTAGCCGATAAAGAATTTTTTGGCTAGAGGGCTT86170.26384432541800357No Hit
GTCTGAGGGTGTCGGCAAACAGAGCAGACAGTAGGCTTGTGTGCAGAACG82090.25135175436420926No Hit
ATTATGTACTGAGTAAACACTGGGAAGAGGCATGCCTGCCTCCCAGACTG73670.2255705170424083No Hit
ATTCAAGGGCTTCCCCAATTTACCAGATGTCAAGGTCGTATGTAATGTTC61390.1879703276942235No Hit
GAGTAAACACTGGGAAGAGGCATGCCTGCCTCCCAGACTGGACCTGGGTC60980.18671494677950398No Hit
GTATATAGCTTTATAAGCCTTTTCCAGATGAGGATTTCTTTAAGTTCTCA55310.16935394730033396No Hit
GTGTAAGTACCGCCAGCCTGGCCATATGGGCCTCTGAGATATGGGAGATG55030.16849661399272062No Hit
CAGTTATACTTGAGCAAGTGAAGGGAAAAACTTTGCTGCCTCTTCCAGCA51660.15817799525466014No Hit
CCCTCAGACTGTGGACTGTGTGTTTACCCTGGTTACTCCCTGGCCCAGCC50080.1533401858759849No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA47090.1441850909125425No Hit
GTAATAGCCTTGGAAGACTTCATGCAGTGGCTGGGCCAGGGAGTAACCAG44950.1376326149186406No Hit
ATCCTGATAAGCAAAATACGGAAAGCTAGTGTGGTTGCGCTCAGCCCTGA44830.13726518635823487No Hit
ATTCTATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACA43800.13411142454808583No Hit
GTAGTAACAAGAGCGAAACTATCTCTCAAAACAAAAACAAAAACAAAAAC43680.13374399598768014No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT42760.1309270436912363No Hit
GTAGTAACAAGAGCGAAACCCCATCTCAAAAAAACAAAACCCAAAACACC42610.13046775799072916No Hit
ATTATTAGGCCTTCCAGCACAGTGACCTGCAAAAGGTTGTATATAGCTTT41570.12728337713387963No Hit
ACAACAGCCGGCAGCGATAGTTCTGTGACATTATGTACTGAGTAAACACT41100.12584428193895725No Hit
AACAAGAGCCGTTCAGATCCAAGCCACTGTTAAAAATCCAAGTAGTACCT40360.12357847248312202No Hit
GTTCCTGACTCTGCACATCTCCTCGTTCTGCACACAAGCCTACTGTCTGC40210.12311918678261488No Hit
CTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACGTGCTT40040.12259866298870677No Hit
GTAGTAACAAGAGCTCTCGAGATTGGGAATAGGTAATAAAGAAAATGAAT39550.12109832970038344No Hit
ATATGTGAGGATGTCAGGCGGCACGCCCACAGCCTCCAATGTGACCTCTC39120.11978171069226297No Hit
CTATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAA38880.11904685357145153No Hit
TTATAAGCCTTTTCCAGATGAGGATTTCTTTAAGTTCTCAGAATTGGAAC37930.1161380441349063No Hit
GCCAACTATGTGTGTGAATGAGTTGGTTCTCTATCTGGGAGGATGCCACG37750.11558690129429773No Hit
TTATTAGGCCTTCCAGCACAGTGACCTGCAAAAGGTTGTATATAGCTTTA37690.11540318701409487No Hit
GTTCAGATCCAAGCCACTGTTAAAAATCCAAGTAGTACCTAGCGCAAGTT37560.11500513940698867No Hit
GTATAACTGAGAGGTCACATTGGAGGCTGTGGGCGTGCCGCCTGACATCC37490.11479080608008535No Hit
CATGATATGTGAGGATGTCAGGCGGCACGCCCACAGCCTCCAATGTGACC37430.11460709179988249No Hit
ACAACAGCTCTCGAGATTGGGAATAGGTAATAAAGAAAATGAATCCTGAG37010.11332109183846248No Hit
GTCCTGGCCAATGAGAAGAATCGCCTAAACTGGCCCCACATGATATGTGA36770.11258623471765104No Hit
TTCCAGCACAGTGACCTGCAAAAGGTTGTATATAGCTTTATAAGCCTTTT35800.10961618718770487No Hit
GTAGTAACAAGAGCCAAAATAAAACTCTTTTCCTTATAAATTACACAATC35630.10909566339379675No Hit
GTAGAAACACGAGCCAAAATAAAACTCTTTTCCTTATAAATTACACAATC34590.10591128253694723No Hit
CTTTATAGCAACGCAAAAAATGGATGAACACAGCTGTGTCCTGTGAACTC34410.10536013969633866No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA124250.0113.325191
TAGTAAC127700.0109.9678652
AGTAACA136450.0102.969193
GCACGCA22100.0100.710132
AGCACGC23350.095.6395341
TAACAAG147200.095.350855
GTAACAA151350.092.927944
CACGCAC25500.087.850723
ACAGCCG9500.084.708394
CGAGCCG7650.082.448895
CAGCCGG9250.077.5926365
AACAAGA183850.076.185196
ACAAGAG197300.070.907277
CAAGAGC192250.068.848588
ACAACGC28050.066.414188
AAGAGCC111050.066.1228269
AACAACG28400.066.106177
AAGAGCG25300.065.6108259
AAACACG48350.064.3261955
CGCACTG2500.063.798395
Produced by FastQC (version 0.11.5)