FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n01_FluA_MK1069.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n01_FluA_MK1069.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2777508
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCCAGA248030.8929947276479491No Hit
ATGTACAGGTATCCTCAGGATTCCAGCCTGTCGGTTTAGGATGCTCGCAA145480.5237788694037965No Hit
AGTAGAAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT142700.513769897332429No Hit
GTATCGGAAGGTGCGGCTGGATCACCTCCTTTCCAGAGCTTCTCGCAAAG135560.48806340071747767No Hit
GTAGAAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTC116410.41911670461435213No Hit
AGCGAAAGCAGGTGCTCTCCCAGCTGAGCTATGCCCCCATGTACAGGTAT101170.3642473757051285No Hit
GTACAGGTATCCTCAGGATTCCAGCCTGTCGGTTTAGGATGCTCGCAAAC97550.35121410991435487No Hit
AGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCCAGAGCTTC97260.3501700085112266No Hit
TATCAAGACGGTGCTCTAACCGACTGAGCTACAGACCCCTGAGTCTGTCT97220.3500259945245882No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC92990.3347965154375793No Hit
TTCCAGAGCTTCTCGCAAAGTTGAGCGCTCACGCTTATCGGCTGTAAATT90180.3246795328762329No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA90120.32446351189627537No Hit
GTCTTATTGTGTTGCCCAGGCTGTGTTACTATTATCACCTGAAAGGTTAT88440.3184149244574633No Hit
GAAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCCAG85220.3068217985330735No Hit
AAGTAATAGTGACTAAGTAAAGGGCTTTCACCGAAGAGGGAGCAATTGTT84200.3031494418737948No Hit
GAGCAAAAGCAGGTGCTCTCCCAGCTGAGCTATGCCCCCATGTACAGGTA76160.2742026305594799No Hit
CAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCCAGAGCT73310.26394163401149523No Hit
ATCGGAAGGTGCGGCTGGATCACCTCCTTTCCAGAGCTTCTCGCAAAGTT73180.2634735885549205No Hit
CTATTACTTATCCATGATCGCCTGGTCCATTCTGATACAAAGAGGGCCTG71590.25774903258604476No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT66230.23845115837650152No Hit
ATTAAAGACAGACTCAGGGGTCTGTAGCTCAGTCGGTTAGAGCACCGTCT65190.2347067947239036No Hit
CCCATGTACAGGTATCCTCAGGATTCCAGCCTGTCGGTTTAGGATGCTCG63250.22772211637194203No Hit
ACTCAGGGGTCTGTAGCTCAGTCGGTTAGAGCACCGTCTTGATAAGGCGG62470.22491384363249356No Hit
GTTTAGGATGCTCGCAAACGCCAAGGCGCTTACAACCACCAACACGTCAG62380.22458981216255722No Hit
GTAGAAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTT61620.22185354641642796No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC60790.21886525619368152No Hit
GACATAATGGATCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCT60730.21864923521372395No Hit
GGTTTAGGATGCTCGCAAACGCCAAGGCGCTTACAACCACCAACACGTCA58130.2092883260822291No Hit
TCTCCCAGCTGAGCTATGCCCCCATGTACAGGTATCCTCAGGATTCCAGC57830.20820822118244126No Hit
AGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC52750.18991844487936668No Hit
GGTCCATGCTCATACCCAAGCAGAAAGTGGCAGGCCCTCTTTGTATCAGA50050.1801975007812759No Hit
GAATAGGAGTGCCTTTTTTTTTCTTTCTTCTCCCTTTTCTTTTCTACTTC49560.17843332944495569No Hit
CTCCCAGCTGAGCTATGCCCCCATGTACAGGTATCCTCAGGATTCCAGCC48750.17551704621552844No Hit
ATTCTGGACAGTGTTCATTGCCCAATCAGAACATCTCAATCCAAAACGGG48430.1743649343224214No Hit
GTTCTTATCTCTTGCTCCACTTCAAGCAATAGATGTAAGGCTTGCATAAA46060.16583210561409725No Hit
GTCTTGATAAGGCGGGGGTCGTTGGTTCGAATCCAACCAGACCCACCATT45930.1653640601575225No Hit
AGCAGGTGCTCTCCCAGCTGAGCTATGCCCCCATGTACAGGTATCCTCAG44930.16176371049156293No Hit
AAACTATTCTCTGTTATCTTCAGTTTGTGTCTCACTTCTTCAATCAACCA44340.1596395041886468No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT44200.15913545523541245No Hit
CCTTTCCAGAGCTTCTCGCAAAGTTGAGCGCTCACGCTTATCGGCTGTAA42660.15359091674983474No Hit
TCTCTGTAGAGTTTCAGAGACTCGAACTGTGTTATCATTCCATTCAAGTC42640.15351890975651555No Hit
TGATAACACAGTTCGAGTCTCTGAAACTCTACAGAGATTCGCTTGGAGAA42120.15164672793021658No Hit
GGATTCCAGCCTGTCGGTTTAGGATGCTCGCAAACGCCAAGGCGCTTACA41810.15053061953376912No Hit
CCTCAGCAGTATGTCCTGGAAGAGAAGGCAATGGTGAAATTTCGCCAACA41310.14873044470078933No Hit
CTCTTGCTCCACTTCAAGCAATAGATGTAAGGCTTGCATAAATGTTATTT41290.14865843770747014No Hit
CTGTAGCTCAGTCGGTTAGAGCACCGTCTTGATAAGGCGGGGGTCGTTGG40980.14754232931102268No Hit
GTGTTACTATTATCACCTGAAAGGTTATAATTAAGAAACTTATTCTTAAT40410.14549013000142574No Hit
GATACAAAGAGGGCCTGCCACTTTCTGCTTGGGTATGAGCATGGACCAGT40410.14549013000142574No Hit
GTTTTGGAGTGAGTGGAGGTCTCCCATTCTCATTACTGCTTCTCCAAGCG40300.14509409153817018No Hit
TCTTATTTCTTCAAACTTCTGACCTAATTGTTCCCGCCATTTCTCGTTTC40190.14469805307491462No Hit
ATTCCAGCCTGTCGGTTTAGGATGCTCGCAAACGCCAAGGCGCTTACAAC40130.14448203209495705No Hit
TCTCATTACTGCTTCTCCAAGCGAATCTCTGTAGAGTTTCAGAGACTCGA39990.14397798314172272No Hit
GTAAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATTTCACCAT39440.14199779082544497No Hit
GAGCGAAAGCAGGTGCTCTCCCAGCTGAGCTATGCCCCCATGTACAGGTA38850.13987358452252882No Hit
CTCCTATTGTGACTGGGTGTATATTCTGGTAAGGGAGACTGCTGTTTATA38450.138433444656145No Hit
CTTTGTATCAGAATGGACCAGGCGATCATGGATAAGTAATAGTGACTAAG37900.13645325233986724No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA37740.13587719639331372No Hit
CCTCAGGATTCCAGCCTGTCGGTTTAGGATGCTCGCAAACGCCAAGGCGC37630.13548115793005816No Hit
CAATAGGAGAGTGCCCAAAATACGTCAGGAGTGCCAAATTGAGGATGGTT37330.1344010530302703No Hit
TTTCCAGAGCTTCTCGCAAAGTTGAGCGCTCACGCTTATCGGCTGTAAAT37230.13404101806367433No Hit
GAGTGGAGGTCTCCCATTCTCATTACTGCTTCTCCAAGCGAATCTCTGTA37130.1336809830970784No Hit
GAACTAGGTGATGCCCCATTCCTTGATCGGCTTCGCCGAGATCAGAAATC36920.13292490966722686No Hit
ATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATCT36510.13144876630418345No Hit
CCCCTGAGTCTGTCTTTAATTTACAGCCGATAAGCGTGAGCGCTCAACTT36440.13119674182756627No Hit
ACCTAACTGACATGACTCTTGAGGAAATGTCAAGGGACTGGTCCATGCTC36140.13011663692777842No Hit
CTGTACCTGCGTCGCGTTACCTAACTGACATGACTCTTGAGGAAATGTCA36100.12997262294114004No Hit
CATTACTGCTTCTCCAAGCGAATCTCTGTAGAGTTTCAGAGACTCGAACT36040.12975660196118247No Hit
CAATATGTATAGGCTACCATGCGAACAATTCAACCGACACTGTTGACACA36000.12961258797454409No Hit
GTAATAGTGACTAAGTAAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGG35460.12766839915492592No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG35030.12612024879856332No Hit
GTGCGGCTGGATCACCTCCTTTCCAGAGCTTCTCGCAAAGTTGAGCGCTC34810.1253281718720522No Hit
GACAATGGTGGGTCTGGTTGGATTCGAACCAACGACCCCCGCCTTATCAA34810.1253281718720522No Hit
CGCTTATCGGCTGTAAATTAAAGACAGACTCAGGGGTCTGTAGCTCAGTC34700.12493213340879665No Hit
GTGCTCTCCCAGCTGAGCTATGCCCCCATGTACAGGTATCCTCAGGATTC34690.12489612991213707No Hit
GAGCTACAGACCCCTGAGTCTGTCTTTAATTTACAGCCGATAAGCGTGAG34630.12468010893217948No Hit
CTTTTTAATGACCGGCTCTCACAATAACTCATGAAAACTCACTATCAGGA34250.12331197605911487No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC34230.12323996906579568No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT34200.12313195857581688No Hit
GCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATCTTCAGTTTGTGT34120.12284393060254012No Hit
GTCTTTAATTTACAGCCGATAAGCGTGAGCGCTCAACTTTGCGAGAAGCT34020.12248389563594415No Hit
ATGTATAGGCTACCATGCGAACAATTCAACCGACACTGTTGACACAGTAC33980.12233988164930577No Hit
AGTAATAGTGACTAAGTAAAGGGCTTTCACCGAAGAGGGAGCAATTGTTG33770.12158380821945428No Hit
GAATGGACCAGGCGATCATGGATAAGTAATAGTGACTAAGTAAAGGGCTT33490.1205757103129856No Hit
GGGTAGAGGCTTTGGGTCCGGCATCATCACCTCAAACGCATCAATGCATG33380.12017967184973005No Hit
GGTTGGATTCGAACCAACGACCCCCGCCTTATCAAGACGGTGCTCTAACC33240.1196756228964957No Hit
GGTATCCTCAGGATTCCAGCCTGTCGGTTTAGGATGCTCGCAAACGCCAA32640.11751541309691997No Hit
CTGCTGAGGATGTCAAAAATGCAGTTGGAGTCCTCATCGGGGGACTTGAA31910.11488715784076949No Hit
CTGTTAACCTGCTCGAAGACAGCCACAACGGAAAACTATGTAGATTAAAA31650.11395106692762No Hit
CATGTACAGGTATCCTCAGGATTCCAGCCTGTCGGTTTAGGATGCTCGCA31570.11366303895434325No Hit
CTACAGAGATTCGCTTGGAGAAGCAGTAATGAGAATGGGAGACCTCCACT31510.11344701797438568No Hit
CTTAAAATGACCATGGCCTCTGTACCTGCGTCGCGTTACCTAACTGACAT30870.11114279418817155No Hit
CTCCAGCACTGGGGATTACAATTCAACATGAGATCTGAGTGTGGACAAAT30520.10988267180508571No Hit
GTTAGAGCACCGTCTTGATAAGGCGGGGGTCGTTGGTTCGAATCCAACCA30490.10977466131510694No Hit
TAATAAATGTTATTGATCTCTTGAAAAAGAAGGAAGAGAAGGAGGGAAGG30470.10970265432178772No Hit
CTGTTATCTTCAGTTTGTGTCTCACTTCTTCAATCAACCATCTTATTTCT30200.10873055991197865No Hit
GGTCCATTCTGATACAAAGAGGGCCTGCCACTTTCTGCTTGGGTATGAGC30070.10826251445540391No Hit
GTTATCTTCAGTTTGTGTCTCACTTCTTCAATCAACCATCTTATTTCTTC29950.10783047249548876No Hit
GTCCAGAATAAACAGAAATCCATTTTGCACTAAGTGGCTGCACAGACCCT29340.10563425919925343No Hit
CTTATCGGCTGTAAATTAAAGACAGACTCAGGGGTCTGTAGCTCAGTCGG29270.10538223472263628No Hit
GTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCCGCAAACGAGT29170.10502219975604031No Hit
AGACAATGGTGGGTCTGGTTGGATTCGAACCAACGACCCCCGCCTTATCA28960.1042661263261888No Hit
ATCCATGATCGCCTGGTCCATTCTGATACAAAGAGGGCCTGCCACTTTCT28950.1042301228295292No Hit
GTCCATGCTCATACCCAAGCAGAAAGTGGCAGGCCCTCTTTGTATCAGAA28830.10379808086961406No Hit
CCCTAAGAGGAAGGGGCAGCACCCTCGGTCTGGACATCGAGACAGCCACA28640.10311401443308173No Hit
GGTTAGAGCACCGTCTTGATAAGGCGGGGGTCGTTGGTTCGAATCCAACC28640.10311401443308173No Hit
ATCAGGAAGACAGCACTAAAGCACAAGGGATCCGACCCCATGATCCAAAC28490.1025739619831878No Hit
GGTACAGAGGCCATGGTCATTTTAAGTGCCTCATCGGATTCTTCTTTCAG28470.10250195498986861No Hit
CACAATAACTCATGAAAACTCACTATCAGGAAGACAGCACTAAAGCACAA28440.10239394449988984No Hit
CAATAGATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTG28390.10221392701659185No Hit
GAAAGCAGGTGCTCTCCCAGCTGAGCTATGCCCCCATGTACAGGTATCCT28070.1010618151234848No Hit
AAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCTAGA27910.10048575917693127No Hit
ATTATGAATAGTCTCCTATTTGAAGTTGTAAACAACCACCTCTGGCCAAA27860.10030574169363328No Hit
GTAAAATAAAGAAAGGAAAATGTCACGTCAGAAATTATGAATAGTCTCCT27860.10030574169363328No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGTTC100.007086938145.031461
CGCAGTA100.0070949537144.97664145
GAGCGAA6750.0132.139771
AGCGAAA28450.0109.856871
AGCAAAA21850.0106.1709442
TATTAGT2850.083.965581
GCAAAAG27800.083.186533
ATTAGTA6200.080.7029951
CTTATTG19900.073.952023
CTAATAA4400.069.193399
GACAGGT451.1929491E-464.458431
ACTAATA5150.060.5242278
GTGTTGC25350.057.475949
AGCAGGG22800.056.591767
GCGAAAG57200.054.8780862
GTCAGAT400.006053717354.371136
TAACTCT400.006055873454.366238145
AAGGGTG30100.054.19055
CGAAAGC58950.053.125993
GAGCAAA44200.052.664141
Produced by FastQC (version 0.11.5)