FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n01_FluA_CM554145.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n01_FluA_CM554145.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2856811
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA57410.20095834131134332No Hit
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC54540.19091217444906225No Hit
GTCTAGAAGGAGTGTACAGAAAGAGAAAAGGATGGTTTGAGGACAGAGCC39320.13763598641982266No Hit
GAGCAAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCAGAGAC36990.12948003910654224No Hit
ATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGGCA33970.11890881125842766No Hit
GGACCAATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCAC31700.11096288833948063No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA12100.097.065911
GAGCAAA64250.072.556271
AGCGAAA21050.071.2943951
CGAAAGC23000.065.55593
GCGAAAG23450.064.29792
CAAAAGC72800.063.6276324
TCACGCA6300.063.284662
CGCAGTC6750.057.9917565
GCAAAAG83450.055.2467963
AGCAAAA84600.054.5814862
GTCACGC7950.049.245181
GCAGTCG12950.048.6996466
AAAGCAG120350.047.824536
AAAAGCA98900.046.9827045
CAGTCGG13450.046.8859637
GTCGGCT13500.046.712319
CGTGTAG21150.044.2135735
TATCGTG21150.044.2135732
CGACGGG2000.043.4938165
ACGCAGT9200.042.54834
Produced by FastQC (version 0.11.5)