FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n01_FluA_CM553981.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n01_FluA_CM553981.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4617774
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA224370.48588345813372413No Hit
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC179760.3892784705358036No Hit
GGACCAATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCAC143160.3100195029033469No Hit
GAGCAAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCAGAGAC139790.30272161435358247No Hit
GTCTAGAAGGAGTGTACAGAAAGAGAAAAGGATGGTTTGAGGACAGAGCC119000.2576999220836706No Hit
ATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGGCA108560.2350916263983469No Hit
GAACCAGTTAAGAGGCTGTTTTTGATCTGGGACAGAGAGAGGGTGATGAC101690.2202143283755333No Hit
GAGCAAAAGCAGGACAGGCAAGGCTGGCAAGAAGGAACCAGTTAAGAGGC100980.21867679102528623No Hit
CACTCTCTAGAAGGAGTGTACAGAAAGAGAAAAGGATGGTTTGAGGACAG98330.21293809528140614No Hit
GGATTGCAGTGGTACGATCTCAGCTCACTGCAGCCCCCACCTCCCATACT98170.21259160799120963No Hit
GTCACGCAGTCGGCTCTGGTTCTCCTCCCACCTCTCTGACCATTCCTTCT87280.18900881680220816No Hit
GTTAAGATACTTGTGGATAGTCGCACAGCTAGAAGATAACAGAGCTAGGA84180.18229562555465037No Hit
ACACAATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA82510.17867916446322407No Hit
AAACATGGCTACAGTCATGTTAAGGGCAGAGGCTCACTGGGGTAAGTGGC82380.17839764353993937No Hit
CTAGAAAAAGAAGGGGCAGCCTCTGGGAAGGGAGGCAAAGGCAGCCAGGT81540.17657858526640757No Hit
GCCAACAGAACATGTGGGCAAAGCGCCGATGAGCTGTTCTGGAGCACGGG79740.1726806032516966No Hit
ACACGATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA76440.16553430289139312No Hit
GAGGCAAGAGAGTCACTTGAGTATGGGAGGTGGGGGCTGCAGTGAGCTGA76140.16488463922227464No Hit
CTTTCTCTAGACCCTGGACCCTGCATAAGATGCAATAAACATGGCTACAG74450.16122486721957377No Hit
TCCCACCTGCAGTCTGGATTCTGTCCCAATCACACACTAAAGAAGAGCCC72590.15719695247103907No Hit
CTGCACTCCATCCTAGGAGACAGAGTGAGACCCTGTCTCAAAAACAAGAA68660.14868635840558675No Hit
GGTGTGAGGAGAACCAGAGCTGACTGCATGACAGAGGGGGGCAGTGGTTC66260.1434890490526388No Hit
GCAGGCAGGATGTGAGGTTGCTCCCTCTCCACCTGCTTCAGCCCTGCCAC65410.1416483353234697No Hit
CTAGACAGTGTTGACAACCACCACCTGTGTTGTGATGAACTTCAACTTTT64300.13924457974773127No Hit
TTGCATGCCCCTCTTCATTCCTCAGGGCTCTGTCCTCAAACCATCCTTTT62990.13640771505924718No Hit
AGAAAAAGAAGGGGCAGCCTCTGGGAAGGGAGGCAAAGGCAGCCAGGTGC62250.1348052113420882No Hit
GGCCAGGCGAGGTGACTCACGCCAGCAATCCCAGCACTTTGGGAGGCCGA61830.1338956822053223No Hit
GTTCGAGACCAGTCTGGCCAACATGGTGAAACCCCATCTCTACTAAAAAT58850.12744235642541188No Hit
CTCTCCACCTGCTTCAGCCCTGCCACTTACCCCAGTGAGCCTCTGCCCTT55920.12109730792368792No Hit
CCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGG53550.11596496493765178No Hit
GAGAGAGGGTGATGACTGATCTGGGGTTGGAGAAGAAAGCACATGTTTGA52680.11408094029720812No Hit
GGGACAGAGAGGGTGATGACTGATCTGGGGTTGGAGAAGAAAGCACATGT52630.11397266301902173No Hit
TCTTTAGTGTGTGATTGGGACAGAATCCAGACTGCAGGTGGGATGTGAGG52290.11323637752735409No Hit
ATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCACACAAGT51960.11252174749132374No Hit
CGTCCTAATAACAAGGGACCAATACATGGGTACCACACTGGGTGCTGAGA51430.11137400834254772No Hit
ATATGGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC51420.11135235288691045No Hit
CAGAACATGTGGGCAAAGCGCCGATGAGCTGTTCTGGAGCACGGGGCCCA50890.11020461373813444No Hit
CTCAAAAGGCTGAGGCAAGAGAGTCACTTGAGTATGGGAGGTGGGGGCTG50840.11009633645994801No Hit
TCCCCACAGGCATCTTGCCTCTCACCCTGTGCTGGGCCCCGTGCTCCAGA50590.10955495006901594No Hit
CTCTCTCACCCCACCTTCCTCTAGCATGCACCTGGCTGCCTTTGCCTCCC50350.10903521913372115No Hit
GGCACGTGCCACTGTGCTCAGCTAATTTTTCTATTTTTAGTAGAGATGGG49940.10814734545259252No Hit
CGATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGG49860.10797410180749425No Hit
GAGTGAGACCCTGTCTCAAAAACAAGAAAGCTAAATTTGTGAACAGTTAA49750.10773589179548415No Hit
CCTTCTAGACAGTGTTGACAACCACCACCTGTGTTGTGATGAACTTCAAC49050.10622000990087432No Hit
GGCAAGGGGCTGGAGCTCAGGAGAGGCTTGGTCTGGAAGGAAAAAGTTGA48010.10396784251459686No Hit
GTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCCTAGCT47240.10230037243052605No Hit
GCCACGTTTATTGCATCTTATGCAGGGTCCAGGGTCTAGAGAAAGAAGGG47140.10208381787415323No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA107800.0111.182341
GAGCGAA29100.0107.888861
AGCAAAA141750.084.133332
CAAAAGC148350.080.536914
CGCAGTC17800.080.236415
AGCGAAA40300.077.534952
TCACGCA18950.075.367192
AAAGCAG201600.074.9432456
GCGAAAG42400.073.523793
CGAAAGC42800.073.006044
GTCACGC19900.072.871651
GCAAAAG166300.071.8439563
AAAAGCA167900.071.634295
ACGCAGT23150.062.006834
CACGCAG23500.061.0833133
TACGACG5900.055.294993
CGACGGG6000.054.373415
GTCGGCT37400.054.2682579
CAGTCGG37400.054.074447
ACGACGG6050.053.924044
Produced by FastQC (version 0.11.5)