FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n01_FluA_CM553980.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n01_FluA_CM553980.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2896083
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA133630.46141633371695495No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT55600.19198344798819647No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA55500.19163815401699466No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT54840.18935921380706283No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG53310.1840762160476754No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC53160.18355827509087275No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG52600.1816246288521427No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT52030.17965645321629248No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT46870.16183928430228No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG45110.1557621104091285No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG44850.15486434608400382No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT41820.14440193875658952No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC41150.1420884691495375No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC41010.14160505758985498No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG39380.13597676585926577No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC39180.13528617791686218No Hit
TCCTTATTCTGGGTCTAGATCCGATATTCGGGATTACAGCTTGTTGGCTT37820.13059017990851782No Hit
TTCTTAGACAGAACCCGACAGAAGAACAAGCTGTGGATATATGCAAGGCT37430.12924353342083084No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA37320.12886371005250885No Hit
ATTATATTCAGTATGGAGAGAATAAAAGAACTACGGAATCTGATGTCGCA36640.12651571104833667No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC35650.12309730073343891No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT35430.12233765399679498No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAGAGAATAAAAGAACTAC35310.12192330123135284No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG35100.12119818389182907No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA34470.11902283187325778No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG34460.11898830247613759No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG34310.1184703615193349No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATATCAAAA34230.11819412634237347No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA33100.11429230446779323No Hit
GATATTGAGAGGATCAGTTGCTCACAAATCTTGCCTACCTGCCTGTGCGT31690.10942365947384795No Hit
GTATACATCGTGGTCATAAGTTCCATTTCTGATTGATCCTATGCAGGCAT30780.10628148433591166No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGG29830.10300119160949461No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG29750.10272495643253317No Hit
ATCTACAAGCCTCCCATTGTAAATGAAGCTAGCAGTTGCATTTTTATCAT29700.10255230944693229No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT29390.10148189813620674No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA29070.10037695742836099No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCC203.8648784E-4108.763631
CGCTCCC700.0103.559376
GAGCGAA13600.093.302141
GCAGGGG12700.082.185229
ATTAGTA1150.075.661661
GCAAAAG89550.070.103513
AGGGTGT11650.068.446976
GCAGGGT26550.066.613199
CAAAAGC99800.064.646784
TAAACTA5000.063.7925725
GCTCCCC1150.063.028527
AAGCAGG122900.061.5130657
AGCAAAA104600.060.8485532
CGAAAGC20850.060.844234
TTTAAAC5050.060.290013
GCTTATA4350.059.993012
AGCGAAA21700.058.126872
AGCAGGT66100.057.5695428
GAGCAAA115900.055.3671651
TAACTCC400.00605482954.3686684
Produced by FastQC (version 0.11.5)