FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n01_FluA_CM553698.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n01_FluA_CM553698.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3348703
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA72850.21754691293912895No Hit
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC58320.1741569795828415No Hit
GGACCAATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCAC54110.1615849479634354No Hit
GAGCAAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCAGAGAC42420.1266759100463672No Hit
TTCTTAGACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT40780.12177849155329691No Hit
GTCTAGAAGGAGTGTACAGAAAGAGAAAAGGATGGTTTGAGGACAGAGCC40190.12001661538810697No Hit
GAGGCAAGAGAGTCACTTGAGTATGGGAGGTGGGGGCTGCAGTGAGCTGA39040.11658244998138086No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA38310.11440250150580689No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT35210.10514518606158862No Hit
GGATTGCAGTGGTACGATCTCAGCTCACTGCAGCCCCCACCTCCCATACT34700.10362220835947529No Hit
ATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGGCA34410.10275620143082262No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA18350.094.426581
CAAAAGC101500.068.490944
GAGCAAA105600.066.526141
GCAAAAG105150.065.561943
AGCGAAA27350.062.5511472
AGCAAAA114850.060.403422
CGAAAGC28450.060.1326454
TCACGCA6050.059.909432
AAAGCAG156700.055.0501566
GCGAAAG32250.053.721583
AAGCAGG164100.052.608737
ATTACGA1900.049.6046331
ACGACGG1900.049.5987054
CGACGGG1650.048.3269425
TACGACG1950.048.3269423
AAAAGCA160050.044.250635
GTCGGCT11750.043.80019
ACGCAGT8000.043.4942474
GCAGTCG12150.042.9572836
CGTGTAG24450.042.693745