FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n01_FluA_CM553445.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n01_FluA_CM553445.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3528127
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA57650.16340114740767553No Hit
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC44270.12547734251062956No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA35630.10098842813764924No Hit
GAGCAAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCAGAGAC35320.100109774959915No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA20350.079.457131
GAGCAAA90200.061.254911
GCAAAAG89550.060.3937233
AGCAAAA92400.058.766312
CGAAAGC27150.058.4780434
CAAAAGC94700.058.104584
AGCGAAA29200.054.3725622
GCGAAAG29800.053.2778133
GTCGGCT9700.050.815269
GCAGTCG10000.049.29786
TCACGCA5200.047.4017222
TATCGTG18100.046.0614742
AAGCAGG154550.045.588397
AAAGCAG154850.045.45976
CGTGTAG19150.043.157345
AAAAGCA136800.041.1768845
ATATCGT20950.039.8021241
ATCGTGT21050.039.26193
ACGCAGT6400.038.51394
ACGACGG2401.8189894E-1236.248384
Produced by FastQC (version 0.11.5)