FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n01_FluA_CM553438.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n01_FluA_CM553438.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2559733
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCCAGA27700.10821441142494159No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA5100.0102.3680041
AGCGAAA19300.099.561291
GCGAAAG20800.092.701012
CGAAAGC23050.083.65213
GTAGCCG16250.049.064978
CATCACG450.00964714448.3253364
ACGCGAT1203.6325218E-948.32156145
TACAGCG604.952482E-448.321567
TAGCCGT19200.041.526349
CAAGGTA26050.039.791884
GAAAGCA53650.036.6155624
GGTAGCC23600.034.39847
ACAAGGT35150.033.6146353
AGGTAGC24800.032.736526
AAGCAGG85800.031.2593966
GAGGCGA1402.7514452E-531.0662866
ACAGCGC1453.4983117E-529.9926958
AAGGTAG35050.029.9879065
AAAGCAG96950.029.9073775
AACAAGG48950.027.6920472
Produced by FastQC (version 0.11.5)