FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n01_FluA_CM552916.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n01_FluA_CM552916.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1946080
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCCAGA41750.21453383211378774No Hit
TTATAATACACTGTAAGAGCAAGACCCCTTTCTGCTGGATTCTGATAAAA26190.13457822905533176No Hit
ATAATACACTGTAAGAGCAAGACCCCTTTCTGCTGGATTCTGATAAAAGA24550.1261510318178081No Hit
AGTAGAAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT23120.11880292690947956No Hit
ATGTACAGGTATCCTCAGGATTCCAGCCTGTCGGTTTAGGATGCTCGCAA21710.11155759269916961No Hit
GAGCAAAAGCAGGTGCTCTCCCAGCTGAGCTATGCCCCCATGTACAGGTA21640.11119789525610459No Hit
GTATCGGAAGGTGCGGCTGGATCACCTCCTTTCCAGAGCTTCTCGCAAAG20680.10626490175121268No Hit
GTTATAATACACTGTAAGAGCAAGACCCCTTTCTGCTGGATTCTGATAAA20130.10343870755570171No Hit
GTAGAAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTC19640.1009208254542465No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA8200.0110.527231
AGCGAAA23700.086.27281
GCGAAAG25300.080.519882
GAGCAAA37100.079.5414661
GGAGCGA1900.072.505861
CGAAAGC28550.071.6077963
CAAAAGC50000.059.0121844
GCAAAAG56950.052.0651253
AGCAAAA58800.050.057142
GTTGCGC450.00964720748.324829
ACGCAGT1109.969517E-846.1341634
AAAAGCA74950.039.5611575
GTAGCCG16700.039.499038
TAGCCGT17000.038.801999
CGACGCC750.001485016438.65986145
AAAGCAG100300.038.2359666
CGCAGTC1458.707302E-734.998335
TCGCTCC2302.7284841E-1134.672265
GTATAAC1751.00932084E-733.1455351
GAAAGCA63450.033.0204934
Produced by FastQC (version 0.11.5)