FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n01_FluA_CM552690.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n01_FluA_CM552690.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3015528
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA73220.24280988271374032No Hit
GGACCAATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCAC53840.17854253052865038No Hit
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC53380.17701709286068643No Hit
GAGCAAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCAGAGAC45080.1494928914604673No Hit
ATCTTGGACAGAGAGAGGGTGATGACTGATCTGGGGTTGGAGAAGAAAGC42440.14073820571389156No Hit
GTCTAGAAGGAGTGTACAGAAAGAGAAAAGGATGGTTTGAGGACAGAGCC41570.13785313882013367No Hit
CACTCTCTAGAAGGAGTGTACAGAAAGAGAAAAGGATGGTTTGAGGACAG38150.126511841375706No Hit
ATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGGCA36360.12057589914602021No Hit
GAGCAAAAGCAGGACAGGCAAGGCTGGCAAGAAGGAACCAGTTAAGAGGC35930.11914994654335824No Hit
ACACAATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA35680.11832090433250826No Hit
GGATTGCAGTGGTACGATCTCAGCTCACTGCAGCCCCCACCTCCCATACT35180.11666281991080832No Hit
CTAGAAAAAGAAGGGGCAGCCTCTGGGAAGGGAGGCAAAGGCAGCCAGGT32530.1078749724757986No Hit
GCCAACAGAACATGTGGGCAAAGCGCCGATGAGCTGTTCTGGAGCACGGG31330.10389556986371871No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA17200.099.479941
GAGCAAA77950.083.896221
TCACGCA6900.073.539672
AGCAAAA87750.073.35652
CAAAAGC94700.068.202524
AGCGAAA24750.067.363622
GCAAAAG99250.065.002823
CGAAAGC25900.064.372574
GCGAAAG26700.062.1723023
GTCGGCT11950.060.6563159
GCAGTCG12350.059.2825856
CAGTCGG12350.058.691747
AAAGCAG143450.056.293396
AAAAGCA124650.052.1642465
CGCAGTC10050.051.2112125
GTCACGC9950.051.0066641
AAGCAGG162400.049.8106357
ACGCAGT10450.048.5572934
CACGCAG11250.047.681723
CGTGTAG24400.042.1862835
Produced by FastQC (version 0.11.5)