FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n01_FluA_CM552654.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n01_FluA_CM552654.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3415479
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA53970.1580159034794241No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC48500.14200058029927867No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT48190.1410929477241699No Hit
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA44200.12941083812841478No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC43770.1281518639113284No Hit
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC43110.12621948488045162No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG40230.11778728547298929No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT38850.11374685659024694No Hit
GGACCAATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCAC35680.10446558154800542No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG35090.10273815180828222No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA34620.10136206371053666No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT34510.10104000053872385No Hit
GTCTAGAAGGAGTGTACAGAAAGAGAAAAGGATGGTTTGAGGACAGAGCC34400.10071793736691106No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG34210.10016164643377985No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA16250.090.128151
TCACGCA3950.066.07292
GCAAAAG83200.061.343323
AGCAAAA86600.058.767492
AGCGAAA24600.058.0562552
GAGCAAA90750.056.9646341
CAAAAGC92100.056.045184
CGAAAGC25800.055.355974
TACGACG1650.052.7248343
GCGAAAG27600.052.008473
GTCGGCT9500.051.120989
CGACGGG1750.049.7119875
ATTACGA1900.045.792051
TTACGAC1900.045.7873572
ACGACGG1900.045.7873574
GCAGTCG10350.045.5292976
AAGCAGG145550.044.8704647
CCCGTAG657.3407526E-444.6133233
ACGCAGT6050.044.3367924
CACGCAG6250.042.918013
Produced by FastQC (version 0.11.5)