FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n01_FluA_CM551963.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n01_FluA_CM551963.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2688440
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA49920.18568389102974217No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA48960.18211304697147787No Hit
TTCTTAGACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT44890.16697415601612833No Hit
ATGTTTGGAGATTGCCTGTAAGCACCTCTTCTTCTTTTTTGACTGACGAC40280.1498266652780051No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG39160.14566068054336342No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT35500.1320468375712309No Hit
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC34250.12739730103703262No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC34010.12650459002246656No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC32120.11947449078275878No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT29010.10790644388567348No Hit
GGACCAATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCAC27420.10199223341417327No Hit
GAGCAAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCAGAGAC26980.10035559655413549No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA17050.094.416721
TCACGCA3150.073.64422
GAGCAAA81600.072.691471
AGCGTCA300.001936786772.4935153
GCAAAAG85300.068.669113
CGAAAGC22650.068.492774
AGCAAAA86000.068.110182
AGCGAAA22800.067.72422
CAAAAGC90200.065.581714
GCAGTCG6600.061.5096446
GCGAAAG26100.060.272383
GTCGGCT6800.059.693889
AAGCAGG131750.056.392837
CCCGTAG651.0947246E-555.7642363
CGTGTAG13500.053.1619035
TATCGTG13950.051.966672
AAAGCAG143500.051.8821146
ATATCGT14250.050.3779681
AAAAGCA126650.047.336865
ACGCAGT4950.046.864494
Produced by FastQC (version 0.11.5)