FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n01_FluA_CM551959.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n01_FluA_CM551959.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2811760
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCCAGA342771.2190585256209634No Hit
AGTAGAAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT227410.8087816883375537No Hit
ATGTACAGGTATCCTCAGGATTCCAGCCTGTCGGTTTAGGATGCTCGCAA197840.7036162403619085No Hit
GTAGAAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTC187830.6680157623694768No Hit
AGCGAAAGCAGGTGCTCTCCCAGCTGAGCTATGCCCCCATGTACAGGTAT170790.6074131504822602No Hit
GTATCGGAAGGTGCGGCTGGATCACCTCCTTTCCAGAGCTTCTCGCAAAG167440.5954989046006771No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT148460.5279966995760662No Hit
TATCAAGACGGTGCTCTAACCGACTGAGCTACAGACCCCTGAGTCTGTCT144240.5129883062565795No Hit
AGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCCAGAGCTTC138690.49324977949753895No Hit
GTACAGGTATCCTCAGGATTCCAGCCTGTCGGTTTAGGATGCTCGCAAAC131940.469243463168976No Hit
AGCGAAAGCAGGCCGGCCTGCTGCCGCGCGACTACCCGGTGAGCATCCAC128270.4561911400688537No Hit
TTCCAGAGCTTCTCGCAAAGTTGAGCGCTCACGCTTATCGGCTGTAAATT124350.4422496941417475No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGT114780.4082140723248072No Hit
AAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCTAGA112000.3983270264887473No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAGCTCTATGCTGACAAAATGACC111930.3980780720971918No Hit
GAAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCCAG109430.3891868438273537No Hit
ATCGGAAGGTGCGGCTGGATCACCTCCTTTCCAGAGCTTCTCGCAAAGTT103160.3668876433265997No Hit
CAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCCAGAGCT101540.36112612740774463No Hit
ATTAAAGACAGACTCAGGGGTCTGTAGCTCAGTCGGTTAGAGCACCGTCT100640.3579252852306029No Hit
AGCGAAAGCAGGCCTATCAGAAACGAATGGGGGTGCAGATGCAACGGTTC97750.34764702535067005No Hit
AAACAAGGTAGTTTTTTACTCCAGCTCTATGCTGACAAAATGACCATCGT97720.34754033061143197No Hit
ACTCAGGGGTCTGTAGCTCAGTCGGTTAGAGCACCGTCTTGATAAGGCGG96640.34369931999886194No Hit
GTTTAGGATGCTCGCAAACGCCAAGGCGCTTACAACCACCAACACGTCAG88000.31297123509830144No Hit
CCCATGTACAGGTATCCTCAGGATTCCAGCCTGTCGGTTTAGGATGCTCG87600.3115486385751273No Hit
GGTTTAGGATGCTCGCAAACGCCAAGGCGCTTACAACCACCAACACGTCA84790.30155489799982926No Hit
TCTCCCAGCTGAGCTATGCCCCCATGTACAGGTATCCTCAGGATTCCAGC83740.2978205821264973No Hit
AGCAGGTGCTCTCCCAGCTGAGCTATGCCCCCATGTACAGGTATCCTCAG81960.29149002759837256No Hit
CTCCCAGCTGAGCTATGCCCCCATGTACAGGTATCCTCAGGATTCCAGCC73200.2603351637408598No Hit
AAGCAGGCCTATCAGAAACGAATGGGGGTGCAGATGCAACGGTTCAAGTG72390.25745440578143225No Hit
GATGAATGCCGCGGCGGGCGACGATAGGGCCTGAAATCGGCTAAAGGTGA71960.2559251145190201No Hit
GGCCTATCAGAAACGAATGGGGGTGCAGATGCAACGGTTCAAGTGATCCT69130.24586024411756335No Hit
GTAGAAACAAGGTAGTTTTTTACTCCAGCTCTATGCTGACAAAATGACCA68640.24411756337667512No Hit
ATTCCAGCCTGTCGGTTTAGGATGCTCGCAAACGCCAAGGCGCTTACAAC64750.23028281218880703No Hit
AGCGAAAGCAGGGATGAATGCCGCGGCGGGCGACGATAGGGCCTGAAATC63630.22629954192391952No Hit
ATCAGAAACGAATGGGGGTGCAGATGCAACGGTTCAAGTGATCCTCTCGC62370.22181836287592113No Hit
GAGCAAAAGCAGGTGCTCTCCCAGCTGAGCTATGCCCCCATGTACAGGTA60130.2138518223461462No Hit
GAGCAAAAGCAGGCCTATCAGAAACGAATGGGGGTGCAGATGCAACGGTT59440.21139784334367087No Hit
GGATTCCAGCCTGTCGGTTTAGGATGCTCGCAAACGCCAAGGCGCTTACA56050.1993413378097704No Hit
CCCCTGAGTCTGTCTTTAATTTACAGCCGATAAGCGTGAGCGCTCAACTT55960.19902125359205622No Hit
CCTTTCCAGAGCTTCTCGCAAAGTTGAGCGCTCACGCTTATCGGCTGTAA55110.19599823598031124No Hit
GGGATGAATGCCGCGGCGGGCGACGATAGGGCCTGAAATCGGCTAAAGGT55000.19560702193643836No Hit
GGTTGGATTCGAACCAACGACCCCCGCCTTATCAAGACGGTGCTCTAACC54690.19450450963097846No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA54550.19400660084786753No Hit
GTCTTGATAAGGCGGGGGTCGTTGGTTCGAATCCAACCAGACCCACCATT53780.19126810254075738No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG52020.18500867783879138No Hit
AAGCAGGGATGAATGCCGCGGCGGGCGACGATAGGGCCTGAAATCGGCTA51460.18301704270634764No Hit
CCTCAGGATTCCAGCCTGTCGGTTTAGGATGCTCGCAAACGCCAAGGCGC51400.1828036532278715No Hit
CTCATAGACTTTGGCACTCCTTCCGTAGAAGGCCCTCCTTTCAGTCCGTA51390.18276808831479216No Hit
CTGTAGCTCAGTCGGTTAGAGCACCGTCTTGATAAGGCGGGGGTCGTTGG51290.18241243918399863No Hit
GGTATCCTCAGGATTCCAGCCTGTCGGTTTAGGATGCTCGCAAACGCCAA51210.1821279198793638No Hit
ATGTACAGGTATCCTCAGGGTGTACCGCCAGATAAGACGGTGGTGGGTCT48600.17284547756565283No Hit
ATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGTTCCTT48460.1723475687825419No Hit
AAAGCAGGCCTATCAGAAACGAATGGGGGTGCAGATGCAACGGTTCAAGT48320.17184965999943097No Hit
GTGCGGCTGGATCACCTCCTTTCCAGAGCTTCTCGCAAAGTTGAGCGCTC47780.16992915469314593No Hit
AGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCTAGAGCTTC47630.16939568099695565No Hit
CACGTATCTCGATCCGCAAGCGCTGAACGGCACCAACGACCTGCGGCTCG47260.1680797792130196No Hit
GAATGGGGGTGCAGATGCAACGGTTCAAGTGATCCTCTCGCTATTGCCGC47000.16715509147295643No Hit
GAAAGCAGGTGCTCTCCCAGCTGAGCTATGCCCCCATGTACAGGTATCCT47000.16715509147295643No Hit
CGCTTATCGGCTGTAAATTAAAGACAGACTCAGGGGTCTGTAGCTCAGTC46370.16491450194895724No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT45960.1634563405127038No Hit
GTGCTCTCCCAGCTGAGCTATGCCCCCATGTACAGGTATCCTCAGGATTC45700.16253165277264064No Hit
GTTCAAGTGATCCTCTCGCTATTGCCGCAAATATCATTGGGATCTTGCAC45690.16249608785956127No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA45350.16128688081486328No Hit
GAGCTACAGACCCCTGAGTCTGTCTTTAATTTACAGCCGATAAGCGTGAG45280.16103792642330783No Hit
GTTTTTTACTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCAC44680.1589040316385467No Hit
CTTATCGGCTGTAAATTAAAGACAGACTCAGGGGTCTGTAGCTCAGTCGG44240.15733917546305518No Hit
GTCTTTAATTTACAGCCGATAAGCGTGAGCGCTCAACTTTGCGAGAAGCT44210.15723248072381713No Hit
CCACTACGCCGGCGCGCGTCACGTCGACGTCACGCCGCTGGCTGACGCGC43590.15502745611289726No Hit
CTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTTTGGCACTCCTTCCG42730.15196887358807296No Hit
CAGCTGAGCTATGCCCCCATGTACAGGTATCCTCAGGATTCCAGCCTGTC41980.14930150510712153No Hit
GGTTAGAGCACCGTCTTGATAAGGCGGGGGTCGTTGGTTCGAATCCAACC40640.1445358067544883No Hit
GAGCGAAAGCAGGTGCTCTCCCAGCTGAGCTATGCCCCCATGTACAGGTA40470.14393120323213932No Hit
CATGTACAGGTATCCTCAGGATTCCAGCCTGTCGGTTTAGGATGCTCGCA40400.14368224884058384No Hit
TTTCCAGAGCTTCTCGCAAAGTTGAGCGCTCACGCTTATCGGCTGTAAAT40290.14329103479671096No Hit
AGATACGTGATTGCGTGCGCGTCAGCCAGCGGCGTGACGTCGACGTGACG39850.14172617862121945No Hit
GACAATGGTGGGTCTGGTTGGATTCGAACCAACGACCCCCGCCTTATCAA39740.14133496457734657No Hit
CAGGTATCCTCAGGATTCCAGCCTGTCGGTTTAGGATGCTCGCAAACGCC39040.1388454206617919No Hit
GTCTCCGGTTACTCGGGAGGCGGAAGGGCCGCCGTCGAAGCATTCGAATC38590.13724499957322103No Hit
GTTAGAGCACCGTCTTGATAAGGCGGGGGTCGTTGGTTCGAATCCAACCA38150.13568014339772955No Hit
GTTGAGCGCTCACGCTTATCGGCTGTAAATTAAAGACAGACTCAGGGGTC37500.13336842404757163No Hit
AGACAATGGTGGGTCTGGTTGGATTCGAACCAACGACCCCCGCCTTATCA37240.13244373630750847No Hit
GTATCGGAAGGTGCGGCTGGATCACCTCCTTTCTAGAGCTTCTCGCCAAG37120.13201695735055624No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA36810.1309144450450963No Hit
ATGGTGGGTCTGGTTGGATTCGAACCAACGACCCCCGCCTTATCAAGACG36680.13045210117506473No Hit
AGCGAAAGCAGGCCGACGTACGCGCGGCGGCCGAACATCGTGTTCCGGTA36570.13006088713119185No Hit
ATCCTCAGGATTCCAGCCTGTCGGTTTAGGATGCTCGCAAACGCCAAGGC36440.12959854326116027No Hit
GAAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCTAG36420.12952741343500157No Hit
CCTGTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGAT35680.12689560986712947No Hit
GCGTTCGACAGTCCGAGCATCAAGTCGAGATTCTGCACGGCCGCACCGGA35550.12643326599709792No Hit
CCACAGCACTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTTTGGC34870.12401485190770194No Hit
ACCCCCGCCTTATCAAGACGGTGCTCTAACCGACTGAGCTACAGACCCCT34070.12116965886135374No Hit
AGGTAGTTTTTTACTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCA33820.12028053603436993No Hit
AGCAAAAGCAGGCCTATCAGAAACGAATGGGGGTGCAGATGCAACGGTTC33670.11974706233817964No Hit
CCTCCTTTCCAGAGCTTCTCGCAAAGTTGAGCGCTCACGCTTATCGGCTG33570.11939141320738614No Hit
GCTCTAACCGACTGAGCTACAGACCCCTGAGTCTGTCTTTAATTTACAGC33490.11910689390275131No Hit
GGGTGTACCGCCAGATAAGACGGTGGTGGGTCTGGTTGGATTCGAACCAA33260.1182889009019262No Hit
AAATTAAAGACAGACTCAGGGGTCTGTAGCTCAGTCGGTTAGAGCACCGT33230.11818220616268815No Hit
TCCTCAGGGTGTACCGCCAGATAAGACGGTGGTGGGTCTGGTTGGATTCG33190.11803994651037072No Hit
CAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCTAGAGCT33150.11789768685805332No Hit
GCGAAAGCAGGTGCTCTCCCAGCTGAGCTATGCCCCCATGTACAGGTATC32860.1168663043787521No Hit
GGTGCTCTCCCAGCTGAGCTATGCCCCCATGTACAGGTATCCTCAGGATT32840.11679517455259339No Hit
CTTTAAATACGGACTGAAAGGAGGGCCTTCTACGGAAGGAGTGCCAAAGT32710.1163328306825618No Hit
TTCTAGAGCTTCTCGCCAAGTTGAGCGCTCACGCTTATCGGCTGTAAATT32530.11569266224713347No Hit
CTAACCGACTGAGCTACAGACCCCTGAGTCTGTCTTTAATTTACAGCCGA31810.1131319885054201No Hit
AAAGCAGGCCGGCCTGCTGCCGCGCGACTACCCGGTGAGCATCCACGCGG31120.11067800950294478No Hit
TTTACGGGGGGCGTGTGCAGGCACCGGCGCGACGCGGATCGGCCTGCCGG30660.10904202350129456No Hit
GTACAGGTATCCTCAGGGTGTACCGCCAGATAAGACGGTGGTGGGTCTGG30540.10861524454434235No Hit
GAGCGAAAGCAGGCCTATCAGAAACGAATGGGGGTGCAGATGCAACGGTT30380.10804620593507269No Hit
TCATTACTCATGTCAAAGGAAGGCACGATCGGGCTCGCTGCCTTTTCGTC30240.10754829715196176No Hit
GCAGGGATGAATGCCGCGGCGGGCGACGATAGGGCCTGAAATCGGCTAAA29980.10662360941189859No Hit
ATCAAGACGGTGCTCTAACCGACTGAGCTACAGACCCCTGAGTCTGTCTT29750.10580561641107349No Hit
TTTTACTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGC29720.10569892167183544No Hit
TGGTGGGTCTGGTTGGATTCGAACCAACGACCCCCGCCTTATCAAGACGG29700.10562779184567672No Hit
GTCTGTAGCTCAGTCGGTTAGAGCACCGTCTTGATAAGGCGGGGGTCGTT29580.1052010128887245No Hit
TTTTTACTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAG29560.1051298830625658No Hit
TATTTAAAGCGACGGTAAATGCATTTGAAAAAAAGACGATCAAGAATCCA28760.1022846900162176No Hit
GATCACCTCCTTTCCAGAGCTTCTCGCAAAGTTGAGCGCTCACGCTTATC28640.10185791105926537No Hit
ACGTGATTGCGTGCGCGTCAGCCAGCGGCGTGACGTCGACGTGACGCGCG28460.10121774262383702No Hit
GCACTTGACATTGTGGATTCTTGATCGTCTTTTTTTCAAATGCATTTACC28250.10047087944917063No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA14650.0138.59121
GAGCAAA23950.0122.318211
AGCGAAA84250.0119.377321
GCGAAAG93100.0107.992912
CGAAAGC95550.0104.844543
GAAAGCA102450.097.7125244
AGTAGAA65900.094.2997741
TATTAGT3550.085.789911
AGCAAAA35050.083.759772
GAGTGGT353.4420034E-582.839467
GCCTAAG300.00193733872.4883962
TAGAAAC90200.068.952383
AGAAACA94150.066.0595254
GTCTATC554.0686664E-665.895037
CGAAAGT451.1951795E-464.434133
GTATAAG451.1951795E-464.434133
TAAGTCG451.1954951E-464.43069145
GTAGAAA99550.062.6946332
GCAAAAG46850.062.3539473
ACTAATA5850.055.757338
Produced by FastQC (version 0.11.5)