FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n01_FluA_CM551950.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n01_FluA_CM551950.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3779501
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA137990.3651011072625725No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG106620.2821007323453546No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC80120.211985656307539No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT77580.20526519241561253No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT72490.19179780611250002No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC70010.18523609333613086No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA60450.15994174892399818No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG58200.15398858209059874No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG55100.1457864411201373No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC54820.1450456025808698No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT52730.13951577205562324No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT51620.13657887641781283No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG49260.13033466587255832No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA49190.13014945623774143No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA48650.12872069619772558No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC48430.12813860877401542No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT47810.12649818057992313No Hit
GTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTTTT47460.12557213240583875No Hit
TCCTTATTCTGGGTCTAGATCCGATATTTGGGATTACAGCTTGTTGGCTT45850.12131231080505071No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTGATGTCGCA44670.11819020553242346No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA43360.11472413950942202No Hit
TTCTTAGACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT41430.10961764529232827No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG41240.10911493342639676No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATATCAAAA41090.10871805563750347No Hit
GAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAACGATT40940.10832117784861017No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA40550.10728929559748761No Hit
TTCTTAATCTGTGTCTCACTTCTTCAATTAGCCATCTTATCTCTTCAAAC40430.10697179336637297No Hit
ATCTACAAGCCTCCCATTGTAAATGAAGCTAGCAGTTGCATTTTTATCAT40410.10691887632785387No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG40260.10652199853896056No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT39860.10546365776857845No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG39760.10519907257598292No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA39330.10406135624782212No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC39210.1037438540167075No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGTG256.469236E-6115.992725
GAGCGAA19850.093.877991
GCCGTAG406.123064E-790.619313
GCAGGGG16400.076.4647459
CGCTCCC406.667656E-572.4954456
GCAAAAG114000.070.524083
CGAAAGC28150.065.928374
GCAGGGT43100.065.75959
AGCGAAA29750.062.3826372
GCTTATA7350.062.1389542
CAAAAGC131850.061.80124
AGCAAAA134500.060.3679582
AAGCAGG168150.058.799897
GAGCAAA139050.058.6119961
AGCCGTA502.0115865E-457.996362
TGTCGAG502.0115865E-457.996362
TAAACTA6000.054.3715825
CTATCAG553.2182023E-452.733731
AGCAGGG78950.052.2419248
TAGCCGT701.6956614E-551.7920571
Produced by FastQC (version 0.11.5)