FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n01_FluA_CM551457.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n01_FluA_CM551457.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2786150
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCCAGA246030.8830464978554636No Hit
TATCAAGACGGTGCTCTAACCGACTGAGCTACAGACCCCTGAGTCTGTCT203120.7290346894460098No Hit
ATGTACAGGTATCCTCAGGATTCCAGCCTGTCGGTTTAGGATGCTCGCAA198420.712165533083287No Hit
AGTAGAAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT186900.6708181540835921No Hit
AGCGAAAGCAGGTGCTCTCCCAGCTGAGCTATGCCCCCATGTACAGGTAT168660.6053514706674085No Hit
GTAGAAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTC157480.5652244136173573No Hit
AAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCTAGA156970.5633939306928917No Hit
GTACAGGTATCCTCAGGATTCCAGCCTGTCGGTTTAGGATGCTCGCAAAC136620.490354072824507No Hit
GTATCGGAAGGTGCGGCTGGATCACCTCCTTTCCAGAGCTTCTCGCAAAG132840.476786964090232No Hit
AGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCCAGAGCTTC106260.3813865010857276No Hit
ATTAAAGACAGACTCAGGGGTCTGTAGCTCAGTCGGTTAGAGCACCGTCT104250.37417224485401No Hit
TTCCAGAGCTTCTCGCAAAGTTGAGCGCTCACGCTTATCGGCTGTAAATT100100.35927713870394634No Hit
ACTCAGGGGTCTGTAGCTCAGTCGGTTAGAGCACCGTCTTGATAAGGCGG93660.3361628053048113No Hit
TCATACAATGGTCGGAACAGAGGGTTGCCAACCCGCGAGGGGGAGCTAAT90290.3240672612745186No Hit
GTCTTTAATTTACAGCCGATAAGCGTGAGCGCTCAACTTTGCGAGAAGCT89620.3216625091972794No Hit
GTTAAGACCGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATTGGTG87460.31390987563483663No Hit
CCCCTGAGTCTGTCTTTAATTTACAGCCGATAAGCGTGAGCGCTCAACTT86750.31136155626940404No Hit
TCTCCCAGCTGAGCTATGCCCCCATGTACAGGTATCCTCAGGATTCCAGC86370.30999766703156684No Hit
CCCATGTACAGGTATCCTCAGGATTCCAGCCTGTCGGTTTAGGATGCTCG85030.30518816287708844No Hit
AGCAGGTGCTCTCCCAGCTGAGCTATGCCCCCATGTACAGGTATCCTCAG83800.3007734687651419No Hit
GAAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCCAG78630.2822173967661468No Hit
GAGCTACAGACCCCTGAGTCTGTCTTTAATTTACAGCCGATAAGCGTGAG78460.28160723579132496No Hit
ATCGGAAGGTGCGGCTGGATCACCTCCTTTCCAGAGCTTCTCGCAAAGTT78450.28157134396927663No Hit
GTTTAGGATGCTCGCAAACGCCAAGGCGCTTACAACCACCAACACGTCAG76260.27371103494068877No Hit
CTCTAAGGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCA75920.272490712991045No Hit
CAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCCAGAGCT75330.27037309549019256No Hit
CCTTAGAGTGCTCTTGCGTAGCAACTAAGGACAAGGGTTGCGCTCGTTGC75090.26951169176103223No Hit
ATGTACAGGTATCCTCAGGGTGTACCGCCAGATAAGACGGTGGTGGGTCT74520.2674658579042765No Hit
TGTACACACCGCCCGTCACACCATGGGAGTGGGTTTTACCAGAAGTGGCT71410.25630350124724083No Hit
GGTTTAGGATGCTCGCAAACGCCAAGGCGCTTACAACCACCAACACGTCA71170.2554420975180805No Hit
CTCCCAGCTGAGCTATGCCCCCATGTACAGGTATCCTCAGGATTCCAGCC68090.24438741632718985No Hit
CCCTAAACGATGTCAACTAGTTGTTGGGGATTCATTTCCTTAGTAACGTA67430.242018556071999No Hit
AGCGAAAGCAGGCCGGCCTGCTGCCGCGCGACTACCCGGTGAGCATCCAC65810.23620408090016687No Hit
GTATCGGAAGGTGCGGCTGGATCACCTCCTTTCTAGAGCTTCTCGCAAAG65500.23509143441666816No Hit
AGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCTAGAGCTTC65090.23361986971268595No Hit
TTCTAGAGCTTCTCGCAAAGTTGAGCGCTCACGCTTATCGGCTGTAAATT64960.23315327602605745No Hit
GGTTGGATTCGAACCAACGACCCCCGCCTTATCAAGACGGTGCTCTAACC64860.23279435780557398No Hit
GGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCAACTAGTTGTT62440.2241085368698742No Hit
ACCCATAAGGGCCATGAGGACTTGACGTCATCCCCACCTTCCTCCGGTTT62290.22357015953914902No Hit
ATTCCAGCCTGTCGGTTTAGGATGCTCGCAAACGCCAAGGCGCTTACAAC61010.21897600631696068No Hit
TGTCAAGGGTAGGTAAGGTTTTTCGCGTTGCATCGAATTAATCCACATCA60540.2172890906806884No Hit
GAGCAAAAGCAGGTGCTCTCCCAGCTGAGCTATGCCCCCATGTACAGGTA59470.21344866572151536No Hit
ATTGTATGACGTGTGAAGCCCTACCCATAAGGGCCATGAGGACTTGACGT58410.20964413258439066No Hit
CCCTTGACATGGTCGGAATTCCGCTGAGAGGTGGAAGTGCTCGAAAGAGA58390.20957234894029395No Hit
CTGTAGCTCAGTCGGTTAGAGCACCGTCTTGATAAGGCGGGGGTCGTTGG56070.20124544622507762No Hit
GGCCAATACTGACGCTCATGCACGAAAGCGTGGGGAGCAAACAGGATTAG54210.1945695673240852No Hit
TTGTATGACGTGTGAAGCCCTACCCATAAGGGCCATGAGGACTTGACGTC53490.19198535613660428No Hit
GAAAGCAGGTGCTCTCCCAGCTGAGCTATGCCCCCATGTACAGGTATCCT52030.18674515011754572No Hit
TCCTCAGGGTGTACCGCCAGATAAGACGGTGGTGGGTCTGGTTGGATTCG51970.18652979918525564No Hit
GAAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCTAG51940.1864221237191106No Hit
GCTCTAACCGACTGAGCTACAGACCCCTGAGTCTGTCTTTAATTTACAGC50700.18197153778511566No Hit
GTCTTGATAAGGCGGGGGTCGTTGGTTCGAATCCAACCAGACCCACCATT50580.1815408359205355No Hit
CCTCAGGATTCCAGCCTGTCGGTTTAGGATGCTCGCAAACGCCAAGGCGC49990.17942321841968306No Hit
CGCTTATCGGCTGTAAATTAAAGACAGACTCAGGGGTCTGTAGCTCAGTC49890.1790643001991996No Hit
CTAACCGACTGAGCTACAGACCCCTGAGTCTGTCTTTAATTTACAGCCGA49720.17845413922437772No Hit
CCTTTCCAGAGCTTCTCGCAAAGTTGAGCGCTCACGCTTATCGGCTGTAA49040.1760134953250902No Hit
GTACAGGTATCCTCAGGGTGTACCGCCAGATAAGACGGTGGTGGGTCTGG47590.17080918112807997No Hit
GGATATAGGCCGCCACGAACAGGCAGGCAACCCCAAGCAGGATGAACCCG47440.17027080379735476No Hit
CCCATGGTGTGACGGGCGGTGTGTACAAGACCCGGGAACGTATTCACCGC47360.16998366922096803No Hit
TCCTTAGAGTGCTCTTGCGTAGCAACTAAGGACAAGGGTTGCGCTCGTTG46850.16815318629650233No Hit
GTGCTCTCCCAGCTGAGCTATGCCCCCATGTACAGGTATCCTCAGGATTC46820.16804551083035732No Hit
TCCCTATCCGATCCTGAACGTGCTTTTCAGCACGTCGCGCAGATGCAGCG46170.16571254239721478No Hit
CAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCTAGAGCT45570.16355903307431402No Hit
CCCTGGTAGTCCACGCCCTAAACGATGTCAACTAGTTGTTGGGGATTCAT45470.16320011485383054No Hit
GTCATGAATCCTACCGTGGTGACCGTCCTCCTTGCGGTTAGACTAGCCAC45250.16241049476876693No Hit
GGTATCCTCAGGATTCCAGCCTGTCGGTTTAGGATGCTCGCAAACGCCAA44630.16018520180176948No Hit
GAGTATGGCAGAGGGGGGTAGAATTCCACGTGTAGCAGTGAAATGCGTAG44050.15810347612296538No Hit
GATGAATGCCGCGGCGGGCGACGATAGGGCCTGAAATCGGCTAAAGGTGA43740.15699082963946664No Hit
CTTATCGGCTGTAAATTAAAGACAGACTCAGGGGTCTGTAGCTCAGTCGG43590.15645245230874147No Hit
GGACAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACG43470.1560217504441613No Hit
ACCCCCGCCTTATCAAGACGGTGCTCTAACCGACTGAGCTACAGACCCCT43380.15569872404572618No Hit
GTGTACAAGACCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTA43370.15566283222367783No Hit
ATCAAGACGGTGCTCTAACCGACTGAGCTACAGACCCCTGAGTCTGTCTT43010.15437072662993737No Hit
ATGGTGGGTCTGGTTGGATTCGAACCAACGACCCCCGCCTTATCAAGACG42290.15178651544245644No Hit
AGACAATGGTGGGTCTGGTTGGATTCGAACCAACGACCCCCGCCTTATCA42250.15164294815426305No Hit
GTACAAGACCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACT42200.15146348904402132No Hit
ACTAAGGACAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACG41390.14855625145810528No Hit
GAGCGAAAGCAGGTGCTCTCCCAGCTGAGCTATGCCCCCATGTACAGGTA41200.14787430683918668No Hit
GGATTCCAGCCTGTCGGTTTAGGATGCTCGCAAACGCCAAGGCGCTTACA40500.14536187929580247No Hit
GTTGAGCGCTCACGCTTATCGGCTGTAAATTAAAGACAGACTCAGGGGTC39720.14256231717603146No Hit
GGTTAGAGCACCGTCTTGATAAGGCGGGGGTCGTTGGTTCGAATCCAACC39710.1425264253539831No Hit
GTTAGAGCACCGTCTTGATAAGGCGGGGGTCGTTGGTTCGAATCCAACCA39480.14170091344687114No Hit
GTGTGTACAAGACCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGAT38830.13936794501372862No Hit
CCTTTCTAGAGCTTCTCGCAAAGTTGAGCGCTCACGCTTATCGGCTGTAA38750.13908081043734186No Hit
TCTCCCAGCTGAGCTATGCCCCCATGTACAGGTATCCTCAGGGTGTACCG38710.13893724314914846No Hit
AGCGAAAGCAGGGATGAATGCCGCGGCGGGCGACGATAGGGCCTGAAATC38250.13728621933492452No Hit
CTTGTACACACCGCCCGTCACACCATGGGAGTGGGTTTTACCAGAAGTGG38000.1363889237837159No Hit
GGTCGGAACAGAGGGTTGCCAACCCGCGAGGGGGAGCTAATCCCAGAAAA37810.13570697916479732No Hit
ATCGGAAGGTGCGGCTGGATCACCTCCTTTCTAGAGCTTCTCGCAAAGTT37800.13567108734274896No Hit
GTGCGGCTGGATCACCTCCTTTCCAGAGCTTCTCGCAAAGTTGAGCGCTC37300.13387649624033165No Hit
GGGATGAATGCCGCGGCGGGCGACGATAGGGCCTGAAATCGGCTAAAGGT36710.13175887873947922No Hit
ATACTGACGCTCATGCACGAAAGCGTGGGGAGCAAACAGGATTAGATACC36660.13157941962923747No Hit
ACCCTTGACATGGTCGGAATTCCGCTGAGAGGTGGAAGTGCTCGAAAGAG36510.13104104229851227No Hit
GCGAAAGCAGGTGCTCTCCCAGCTGAGCTATGCCCCCATGTACAGGTATC35660.12799023742440285No Hit
CAGGTATCCTCAGGATTCCAGCCTGTCGGTTTAGGATGCTCGCAAACGCC35240.1264827808983723No Hit
GTATGGCAGAGGGGGGTAGAATTCCACGTGTAGCAGTGAAATGCGTAGAG35230.12644688907632395No Hit
GACAATGGTGGGTCTGGTTGGATTCGAACCAACGACCCCCGCCTTATCAA34850.1250829998384868No Hit
ATCCTCAGGATTCCAGCCTGTCGGTTTAGGATGCTCGCAAACGCCAAGGC34620.12425748793137484No Hit
TGGTGGGTCTGGTTGGATTCGAACCAACGACCCCCGCCTTATCAAGACGG34540.12397035335498807No Hit
AAATTAAAGACAGACTCAGGGGTCTGTAGCTCAGTCGGTTAGAGCACCGT34470.12371911060064965No Hit
CCTTAGTAACGTAGCTAACGCGTGAAGTTGACCGCCTGGGGAGTACGGTC34370.12336019238016618No Hit
CAGCTGAGCTATGCCCCCATGTACAGGTATCCTCAGGATTCCAGCCTGTC33780.12124257487931375No Hit
CTTATGGGTAGGGCTTCACACGTCATACAATGGTCGGAACAGAGGGTTGC33720.12102722394702367No Hit
AAGCAGGGATGAATGCCGCGGCGGGCGACGATAGGGCCTGAAATCGGCTA33580.12052473843834682No Hit
TTTCCAGAGCTTCTCGCAAAGTTGAGCGCTCACGCTTATCGGCTGTAAAT32810.11776106814062415No Hit
ACTCTAAGGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTC32190.11553577517362669No Hit
CCCATGTACAGGTATCCTCAGGGTGTACCGCCAGATAAGACGGTGGTGGG32040.1149973978429015No Hit
CTCCCAGCTGAGCTATGCCCCCATGTACAGGTATCCTCAGGGTGTACCGC31720.11384885953735441No Hit
GGTGCTCTCCCAGCTGAGCTATGCCCCCATGTACAGGTATCCTCAGGATT31690.11374118407120938No Hit
TTCCGATACGGCTACCTTGTTACGACTTCACCCCAGTCATGAATCCTACC31340.11248497029951725No Hit
TTCATTTCCTTAGTAACGTAGCTAACGCGTGAAGTTGACCGCCTGGGGAG31270.11223372754517882No Hit
GGGTGTACCGCCAGATAAGACGGTGGTGGGTCTGGTTGGATTCGAACCAA30850.11072627101914828No Hit
TTTCAGCACGTCGCGCAGATGCAGCGCGGCGCGATTGCTGTCGTCCGTCA30790.11051092008685821No Hit
GTCCTTAGTTGCTACGCAAGAGCACTCTAAGGAGACTGCCGGTGACAAAC30640.10997254275613301No Hit
CATGTACAGGTATCCTCAGGATTCCAGCCTGTCGGTTTAGGATGCTCGCA30640.10997254275613301No Hit
CACCATGGGAGTGGGTTTTACCAGAAGTGGCTAGTCTAACCGCAAGGAGG30480.10939827360335948No Hit
CTTCCACCTCTCAGCGGAATTCCGACCATGTCAAGGGTAGGTAAGGTTTT30160.10824973529781239No Hit
GGTATCCTCAGGGTGTACCGCCAGATAAGACGGTGGTGGGTCTGGTTGGA30090.10799849254347396No Hit
CTGCAACTCGAGTGCATGAAGCTGGAATCGCTAGTAATCGCGGATCAGCA29980.10760368250094215No Hit
CCTCCTTTCCAGAGCTTCTCGCAAAGTTGAGCGCTCACGCTTATCGGCTG29920.10738833156865209No Hit
GCGTAGAGATGTGGAGGAATACCGATGGCGAAGGCAGCCCCCTGGGCCAA29740.10674227877178184No Hit
CCACTACGCCGGCGCGCGTCACGTCGACGTCACGCCGCTGGCTGACGCGC29170.10469644491502611No Hit
ACAGACCCCTGAGTCTGTCTTTAATTTACAGCCGATAAGCGTGAGCGCTC29090.10440931033863934No Hit
GTCTGTAGCTCAGTCGGTTAGAGCACCGTCTTGATAAGGCGGGGGTCGTT28920.10379914936381744No Hit
GTCTGGTTGGATTCGAACCAACGACCCCCGCCTTATCAAGACGGTGCTCT28610.1026865028803187No Hit
GTGCTCTAACCGACTGAGCTACAGACCCCTGAGTCTGTCTTTAATTTACA28580.10257882741417368No Hit
CTCCCATGGTGTGACGGGCGGTGTGTACAAGACCCGGGAACGTATTCACC28580.10257882741417368No Hit
GGGGAGTACGGTCGCAAGATTAAAACTCAAAGGAATTGACGGGGACCCGC28470.10218401737164186No Hit
GTCAAGGGTAGGTAAGGTTTTTCGCGTTGCATCGAATTAATCCACATCAT28420.10200455826140013No Hit
ATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATTGG28410.10196866643935179No Hit
CACGTATCTCGATCCGCAAGCGCTGAACGGCACCAACGACCTGCGGCTCG28400.10193277461730343No Hit
TTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGC27920.10020996715898282No Hit
ATTTACAGCCGATAAGCGTGAGCGCTCAACTTTGCGAGAAGCTCTGGAAA27870.1000305080487411No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA10900.0131.683041
AGTAGAA29550.0100.090731
TACGCGC259.3979004E-486.978134145
GCAAAAG26250.082.56763
GAAAGCA73150.081.85284
CAAAAGC27050.080.3936544
AGCGAAA77050.077.7138751
GCGAAAG84450.070.900322
ATTAGTA4400.070.844631
AAAGCAG102700.066.065275
GTAGAAA47450.062.3292272
TAGAAAC47900.061.592343
GGATATA10500.061.445761
AAAAGCA35750.061.032095
AGAAACA51450.057.342524
AAACAAG110050.056.32061
TTAAGAC24800.055.5353782
GATATAG11650.055.377312
GCAGGAC4650.054.556188
GTTAAGA25300.054.440771
Produced by FastQC (version 0.11.5)