FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n01_FluA_CM551087.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n01_FluA_CM551087.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4020545
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA116880.29070685690621545No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA79700.1982318317541527No Hit
GGACCAATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCAC78020.19405329376987449No Hit
TTCTTAGACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT72200.17957764432433912No Hit
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC71850.17870711557761446No Hit
ATGTTTGGAGATTGCCTGTAAGCACCTCTTCTTCTTTTTTGACTGACGAC71370.1775132475821064No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG66790.16612175712496688No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT54300.1350563169918506No Hit
GGATTGCAGTGGTACGATCTCAGCTCACTGCAGCCCCCACCTCCCATACT54280.13500657249203776No Hit
CTCTCCACCTGCTTCAGCCCTGCCACTTACCCCAGTGAGCCTCTGCCCTT50250.12498305577975126No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC49590.12334148728592766No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT49510.1231425092866763No Hit
ATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGGCA44360.11033330058487095No Hit
GAGCAAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCAGAGAC42420.10550808410302584No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG40520.10078235662080638No Hit
GAACCAGTTAAGAGGCTGTTTTTGATCTGGGACAGAGAGAGGGTGATGAC40380.10043414512211651No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA24950.092.705871
CGTTACT259.392496E-486.991444
GAGCAAA116900.076.4158251
CGACGGG1450.069.993115
TACGACG1450.069.993113
ACGACGG1450.069.993114
GCAAAAG126700.069.46043
AGCAAAA128300.068.876692
CAAAAGC131050.067.597324
TCACGCA7000.067.3148042
AGCGAAA34800.066.2434842
CGAAAGC35000.066.0720754
ATTACGA1650.061.5221061
TTACGAC1650.061.50912
GCGAAAG40600.057.1372343
ACGCAGT8800.054.369654
AAGCAGG206550.053.8334547
AAAGCAG213200.052.2275056
GTCGGCT14800.049.956339
CACGCAG10050.048.328583
Produced by FastQC (version 0.11.5)