FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n01_FluA_CM550443.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n01_FluA_CM550443.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3185242
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTACC118180.371023614532271No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG116700.36637718578368617No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG116430.36552952648495785No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT116160.3646818671862295No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC109700.3444008335944333No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT101700.31928500252100156No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA101570.31887687026605827No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTAGCATTTGGCCTGGT80610.25307339285366703No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC77360.24287008648008535No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTAGCATTTGGCCTGGTGTGTG71360.2240332131750115No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG65900.2068916584673943No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGG64830.20353241606132283No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG64760.2033126525394303No Hit
TCCCAGCACAGGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAAT64690.20309288901753775No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA62820.19722206350412308No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA59060.18541762289961014No Hit
TTATTTGGGTCTCCATTCCCATTGAGGGCATTTTGAACAAAGCGTCTACG57230.17967237654156262No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACT56480.1773177673784284No Hit
AAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGT53730.16868420044693622No Hit
TTCCCATTGAGGGCATTTTGAACAAAGCGTCTACGCTGCAGTCCTCGCTC51220.16080410844764698No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTACCAGAAACGAAT50630.1589518159059814No Hit
TGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACGATCAAGAATCCA49250.15461933504581443No Hit
CACCAGGCCAAATGCTACTTCAGTGGTTACAGCCCCCATCCTATTGTATA48670.1527984372929906No Hit
GGCCTGGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTC48470.1521705415161548No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTAGCATTTGGCCTGGTGT48260.1515112509504772No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG47440.1489368782654505No Hit
TCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGAC47340.14862293037703256No Hit
GGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAACGTATGTTCT47140.14799503460019678No Hit
TTCCATGAGAGCCTCAAGATCTGTGTTTTTCCCAGCAAAGACATCTTCAA46290.14532647754864467No Hit
GACTAAGGGGATTTTAGGGTTTGTTTTCACGCTCACCGTGCCCAGTGAGC42410.13314529947803022No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT41710.13094766425910495No Hit
GCTTTGTCCATGTTATTTGGGTCTCCATTCCCATTGAGGGCATTTTGAAC41030.12881281861786326No Hit
GCCTATGAGACCTGTGCTGGGAATCAGCAATCTGCTCACATGTTGCACAC40760.12796515931913494No Hit
CTCATTGCCTGCACCATCTGCCTGGCCTGACTAGCAATCTCCATGGCCTC40650.12761981664187524No Hit
CTCATAGGCAGATGGTGGCAACAACCAATCCATTAATAAAACATGAGAAC40540.12727447396461555No Hit
CAGTTATTCTGCTGGTGCACTTGCCAGTTGCATGGGCCTCATATACAATA40420.12689773649851407No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG40040.1257047345225261No Hit
ATCAAGTGCAAGATCCCAATGATATTCGCGGCAACAACAAGCGGGTCACT39580.12426057423580374No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC39350.12353849409244258No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTAGCATTTGGCCTGG39160.12294199310444857No Hit
TCTATCGACTCTTCAAACACGGCCTTAAAAGAGGCCCTTCTACGGAAGGA38630.12127806929583373No Hit
ATATTGTGGATTCTTGATCGTCTTTTTTTCAAATGCGTCTATCGACTCTT37890.11895485492154129No Hit
CCACAATATCAAGTGCAAGATCCCAATGATATTCGCGGCAACAACAAGCG37080.11641187702535633No Hit
ATCCCAATGATATTCGCGGCAACAACAAGCGGGTCACTTGAATCGTTGCA36950.11600374477041306No Hit
GTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTG36910.11587816561504588No Hit
GAATAACTGAGAGCTATTTCTTTGGCCCCGTGGAACGTTATCTCCCTCTT36640.11503050631631757No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC36420.11433982096179819No Hit
GACTAGCAATCTCCATGGCCTCCGCTGCCTGCTCACTTGATCCAGCCATT35850.11255031799781619No Hit
ATTGAAAGATGAGCCTTCTAACCGAGGTCGAAACGTATGTTCTCTCTATC35760.11226776489824007No Hit
TTCTTAATCTGTGTCTCACTTCTTCAATTAGCCATCTTATCTCTTCAAAC35170.11041547235657448No Hit
CCCAAATAACATGGACAAAGCAGTTAAACTGTATAGGAAACTTAAGAGGG34840.1093794443247954No Hit
CTTCAAACACGGCCTTAAAAGAGGCCCTTCTACGGAAGGAGTACCTGAGT34550.10846899544838351No Hit
GGACAAAGCAGTTAAACTGTATAGGAAACTTAAGAGGGAGATAACGTTCC34420.10806086319344024No Hit
GTCCCAATGGCTCTCATTGCCTGCACCATCTGCCTGGCCTGACTAGCAAT34200.10737017783892089No Hit
CAATGGGAATGGAGACCCAAATAACATGGACAAAGCAGTTAAACTGTATA34170.10727599347239551No Hit
GGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGGGATTTTAGGG34160.10724459868355371No Hit
CCTCTGACTAAGGGGATTTTAGGGTTTGTTTTCACGCTCACCGTGCCCAG32290.10137377317013903No Hit
CTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTTGAA32250.10124819401477188No Hit
GGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTCTCATA32250.10124819401477188No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA20600.091.173311
CGAAAGC20700.090.71153
GCGAAAG24050.078.678912
TAGAAAC26000.060.787752
AGTAGAA25400.053.387931
GAGCGAA10100.053.1307031
GGGTTAA5250.046.962891
GTAGCCG2950.039.3166358
GTAGAAA43300.039.0214351
GCAGTCG1157.1481954E-637.8256456
CAAGGTA26100.037.4948549
AGCAGGG24550.036.3188447
TATATTC5750.035.3039363
TCGTACT7250.034.999592
AAGGTAG26900.034.767255
TATAGGG1051.8547512E-434.5234032
ACAAGGT30750.034.4180378
CGTACTC7400.034.290143
GTAGGAC850.002743037734.117253
AAGCAGG67700.033.9471666
Produced by FastQC (version 0.11.5)