FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n01_FluA_CM550272.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n01_FluA_CM550272.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2004894
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA39860.19881350335728473No Hit
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC31200.15561919981804526No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA28180.14055605932283702No Hit
GGACCAATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCAC26330.1313286388208055No Hit
TTCTTAGACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT22510.11227526243282686No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT21020.10484344808254203No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC20190.10070357834379275No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA9550.0100.994621
AGCGAAA12750.072.2198942
CGAAAGC13050.070.559674
GCAAAAG52550.064.018963
GCGAAAG14500.063.5037043
CAAAAGC54000.062.4341964
GAGCAAA56650.060.4215851
AGCAAAA57900.058.479232
TCACGCA3450.056.742442
GTCGGCT6300.054.0810139
TGTCCGA553.2188566E-452.7303472
CGTGTAG12300.047.746695
GCAGTCG7200.047.329156
TATCGTG12700.046.8137552
AAAGCAG92850.046.383966
TACGACG951.9210856E-645.792143
ATATCGT13100.045.3933831
AAGCAGG95000.045.097397
AAAAGCA76100.044.8744965
ACGCAGT4600.044.1330074
Produced by FastQC (version 0.11.5)