FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n01_FluA_BHHS1178.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n01_FluA_BHHS1178.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2964214
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT43650.14725657459279257No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA42040.14182511788959906No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG37140.12529459748857538No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT37070.1250584471971322No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT37050.12499097568529129No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA36870.12438373207872305No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG34430.11615220763413169No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC33930.11446541983810886No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC33780.113959383499302No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTG32470.1095399994737222No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT31470.10616642388167656No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGACTGCTTTCTTTGGCATATCC30600.10323141311659684No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA9150.085.583161
AGCGAAA18350.067.1735841
CGAAAGC19500.064.313463
GCAAAAG61550.054.1776543
GCGAAAG23700.052.916142
CAAAAGC77500.043.962924
CAGTACG6200.042.086489
AGCAAAA85500.039.0863572
GAGCAAA90800.037.531651
AAGCAGG111500.036.4036567
TCGTACT8450.034.3157652
GCGTACT950.00473443830.518852145
CGTACTC9650.030.0485173
TAGAAAC33300.029.3886742
CAAGGGT29100.028.8971794
AGGGTGT23500.028.071396
AAAAGCA124300.027.7021125
ACTATTC9750.027.5060928
GCAGGGT33250.027.2489769
AAAGCAG156600.026.3397066
Produced by FastQC (version 0.11.5)