FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n01_FluA_BHE1201.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n01_FluA_BHE1201.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3003903
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTTAGACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT47110.156829298416094No Hit
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA33110.11022326619734392No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA15550.079.264721
GCAAAAG73300.063.5921253
GAGCAAA81050.059.6669961
CAAAAGC81650.058.065464
CGAAAGC20950.057.7868164
AGCAAAA84700.055.7177932
TCACGCA4050.055.48852
AGCGAAA22450.053.602872
GCGAAAG25050.049.775663
TACGACG1650.048.3286933
GTCGGCT6600.048.326289
TTAGACA17000.046.05444
AAGCAGG129450.045.6383747
GCAGTCG7000.045.5670556
TATCGTG12300.043.61372
CGTGTAG12250.043.1999365
CGACGGG1903.6379788E-1241.9696545
TTACGAC1903.6379788E-1241.9696542
ACGCAGT5250.041.42464
AAAGCAG144250.040.8071756
Produced by FastQC (version 0.11.5)