FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n01_FluA_BHE1163.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n01_FluA_BHE1163.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3388239
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA174880.5161383243625967No Hit
ATATCGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC132670.3915603356197718No Hit
GGACCAATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCAC121370.3582096776526095No Hit
GAGCAAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCAGAGAC98710.2913312785786363No Hit
GAGGCAAGAGAGTCACTTGAGTATGGGAGGTGGGGGCTGCAGTGAGCTGA95520.28191635832064976No Hit
ATCTTGGACAGAGAGAGGGTGATGACTGATCTGGGGTTGGAGAAGAAAGC89560.26432609978221727No Hit
CACTCTCTAGAAGGAGTGTACAGAAAGAGAAAAGGATGGTTTGAGGACAG86370.2549111795242307No Hit
GGATTGCAGTGGTACGATCTCAGCTCACTGCAGCCCCCACCTCCCATACT84660.24986431004424423No Hit
GTCTAGAAGGAGTGTACAGAAAGAGAAAAGGATGGTTTGAGGACAGAGCC80490.23755703183866309No Hit
ATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGGCA77330.2282306531505009No Hit
GAGCAAAAGCAGGACAGGCAAGGCTGGCAAGAAGGAACCAGTTAAGAGGC75580.22306572824408194No Hit
GAACCAGTTAAGAGGCTGTTTTTGATCTGGGACAGAGAGAGGGTGATGAC75010.22138343841741978No Hit
ACACAATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA73860.21798934490748734No Hit
GCCAACAGAACATGTGGGCAAAGCGCCGATGAGCTGTTCTGGAGCACGGG65800.1942011764813521No Hit
GTCACGCAGTCGGCTCTGGTTCTCCTCCCACCTCTCTGACCATTCCTTCT64560.1905414582619467No Hit
CTAGAAAAAGAAGGGGCAGCCTCTGGGAAGGGAGGCAAAGGCAGCCAGGT64030.18897722386171695No Hit
CTGCACTCCATCCTAGGAGACAGAGTGAGACCCTGTCTCAAAAACAAGAA60930.1798279283132034No Hit
GGTGTGAGGAGAACCAGAGCTGACTGCATGACAGAGGGGGGCAGTGGTTC59960.1769650842222169No Hit
GTTAAGATACTTGTGGATAGTCGCACAGCTAGAAGATAACAGAGCTAGGA59820.17655189022970338No Hit
ACACGATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA59150.17457446183696015No Hit
CTAGACAGTGTTGACAACCACCACCTGTGTTGTGATGAACTTCAACTTTT58070.17138696532328446No Hit
TTGCATGCCCCTCTTCATTCCTCAGGGCTCTGTCCTCAAACCATCCTTTT57900.1708852297609466No Hit
CTTTCTCTAGACCCTGGACCCTGCATAAGATGCAATAAACATGGCTACAG56750.16749113625101417No Hit
GCAGGCAGGATGTGAGGTTGCTCCCTCTCCACCTGCTTCAGCCCTGCCAC53810.15881406240823034No Hit
AAACATGGCTACAGTCATGTTAAGGGCAGAGGCTCACTGGGGTAAGTGGC53480.15784010514016278No Hit
GGGACAGAGAGGGTGATGACTGATCTGGGGTTGGAGAAGAAAGCACATGT51110.15084532112404114No Hit
AGAAAAAGAAGGGGCAGCCTCTGGGAAGGGAGGCAAAGGCAGCCAGGTGC47650.14063352673763568No Hit
TCCCACCTGCAGTCTGGATTCTGTCCCAATCACACACTAAAGAAGAGCCC47150.13915783390723027No Hit
TCCCCACAGGCATCTTGCCTCTCACCCTGTGCTGGGCCCCGTGCTCCAGA45170.13331409029882485No Hit
CAGAACATGTGGGCAAAGCGCCGATGAGCTGTTCTGGAGCACGGGGCCCA45070.13301895173274378No Hit
GGCAAGGGGCTGGAGCTCAGGAGAGGCTTGGTCTGGAAGGAAAAAGTTGA44700.13192693903824376No Hit
GTTCGAGACCAGTCTGGCCAACATGGTGAAACCCCATCTCTACTAAAAAT43770.12918215037368969No Hit
GGCACGTGCCACTGTGCTCAGCTAATTTTTCTATTTTTAGTAGAGATGGG42900.12661444484878429No Hit
CTCTTTCCTCAGCCTCTTGAGTAGCTGGGACTACAGGCATGTGCCACTGT42660.1259061122901897No Hit
CTCTCCACCTGCTTCAGCCCTGCCACTTACCCCAGTGAGCCTCTGCCCTT42380.12507972430516265No Hit
GAGAGAGGGTGATGACTGATCTGGGGTTGGAGAAGAAAGCACATGTTTGA41830.12345646219171669No Hit
CGATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGG39850.11761271858331127No Hit
CCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCAAGG38990.11507452691501396No Hit
CATCTTCTGCTGGCAGAGTCTCCCCGATTCCATCTTCCACAGCCCTCTCA37700.11126723941256801No Hit
CGTCCTAATAACAAGGGACCAATACATGGGTACCACACTGGGTGCTGAGA37660.11114918398613556No Hit
CTCTCTCACCCCACCTTCCTCTAGCATGCACCTGGCTGCCTTTGCCTCCC37000.10920126945000042No Hit
ATATTGTGTAGAATTAGACCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC36300.10713529948743286No Hit
CCTTCTAGACAGTGTTGACAACCACCACCTGTGTTGTGATGAACTTCAAC34900.1030033595622977No Hit
GACCCTTCCTTCTCAGGCTCCTTTGCGTGCCCCTCTTCATTCCTCAGGGC34710.10244259628674364No Hit
ATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCACACAAGT34150.10078982031668958No Hit
ATATGGTGTAGAATTAGGCCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC33980.10028808475435175No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA19450.0114.0738751
GAGCAAA79400.0107.483141
CGCAGTC13500.095.0530855
TCACGCA15050.086.7099842
AGCAAAA101100.084.259682
CAAAAGC104100.081.969524
GTCACGC16400.080.023791
AAAGCAG138650.077.282516
AGCGAAA28800.077.030382
ACGCAGT16900.076.3589554
CACGCAG17600.073.734973
GCAAAAG116350.073.215763
GCGAAAG30350.073.096373
AAAAGCA121850.070.564445
CAGTCGG24650.070.281917
GTCGGCT24700.070.139649
CGAAAGC31650.069.86394
GCAGTCG26450.065.23466
AGTCGGC27350.063.8737348
CGTGTAG48600.055.492385
Produced by FastQC (version 0.11.5)