FastQCFastQC Report
Thu 25 Oct 2018
HMNG2BCX2_l01_n01_FluA_BHE1039.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMNG2BCX2_l01_n01_FluA_BHE1039.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2937735
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA104300.35503542695307777No Hit
GAGCAAAAGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCT66950.22789666188407057No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGACTCCAACACTGTGTCAAG60860.20716640541097137No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA52230.1777900321165796No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA50370.17145862373563306No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATATTATTTGGGTCTCCATTCCC48410.16478681705463563No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT48290.16437833909457455No Hit
GTATAGGAAACTTAAGAGAGAGATAACGTTCCATGGGGCCAAAGAAATAG48090.16369754249447277No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT45030.15328135451291555No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT43570.1483115393321726No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG42840.14582663174180108No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTG42660.14521391480170948No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA42130.14340980381143978No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC37540.12778552183910394No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC35190.11978616178790803No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG35190.11978616178790803No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT35170.11971808212789786No Hit
AGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCTTCAACCC34590.1177437719876027No Hit
CTTTTAAGGCCATGTTTGAAGAGTCGATAGATGCATTTGAAAAAAAGACG34550.11760761266758234No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA33970.11563330252728718No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATATCAAAA33580.11430574915708872No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG33140.1128079966368648No Hit
TTATTTGGGTCTCCATTCCCATTGAGGGCATTTTGGACAAAGCGTCTACG32850.11182084156671722No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA32120.10933593397634572No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACCATCATTGCTTT31630.10766798230609637No Hit
ATATACAGGAGAGTCGACGGGAAATGGATGAGGGAACTCGTCCTTTATGA31560.10742970349606074No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG30580.10409380015556202No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG29960.10198333069524651No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA17650.098.602831
TACGACG353.4388304E-582.8549963
TTACGAC353.4388304E-582.8549962
ACGACGG353.4388304E-582.8549964
AGTAGAA12000.077.952731
CAAAAGC83750.076.5233844
GCAAAAG85050.074.842273
TCACGCA608.038478E-872.4981162
CGAAAGC25550.067.532494
GTCACGC651.3971112E-766.936151
CGCAGTC651.3992576E-766.921345
AGCGAAA26450.064.960512
ATTACGA451.19308825E-464.457031
AAGCAGG126900.063.6353657
GCAGGGG9600.060.40799
GCAGGGT34900.058.5731629
GCGTCAG502.0089476E-458.011331
GCGAAAG31100.055.713993
CGTGTAG2500.055.098575
AGCAGGG48150.054.950498
Produced by FastQC (version 0.11.5)