FastQCFastQC Report
Fri 21 Jul 2017
HMN35AFXX_n02_matalpha2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN35AFXX_n02_matalpha2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7529185
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG57000.017.570711
TACACCG23700.016.7122765
TATACGG43100.016.5416625
CTATACT63800.015.8038634
GTATATG84300.015.64131
GTATAGA61200.015.4230131
GTATATA153900.015.122141
TCTATAC62300.014.5674853
CCTATAC37550.014.3864423
TATACAG77950.014.3197965
TACGGTG39150.014.1618387
ACGGTGT37250.014.1107018
TATACTG91800.014.0420855
TAGGTAG31750.013.8358985
TAGACAG35450.013.8138245
GTCCTAG16000.013.5098521
CTAATAC62950.013.5016973
TATACCG36050.013.1843865
GTATAGG39550.013.1170261
GGTAGTC30800.013.0917577
Produced by FastQC (version 0.11.2)