FastQCFastQC Report
Fri 21 Jul 2017
HMN35AFXX_n02_matalpha1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN35AFXX_n02_matalpha1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4290050
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG17700.018.715955
TCTATAC29400.017.8843753
GTATTAG30550.017.4546591
TACACCG12550.016.6410835
GTATAGA30450.016.5653881
CTATACT32850.016.003684
GTGTAAG19150.015.4278681
GTATATG44950.015.0691591
CTAATAC34200.014.531863
CCTGGAC10950.014.4712753
TATACAG38550.014.3844635
CCGCACG6051.2561031E-714.2812799
TTATACT53100.014.2405634
GTATATA76650.014.007641
TATTAGG42850.013.9532762
TAGACCG14500.013.9064855
TATACGG21100.013.652275
ACCGTCG12900.013.3957738
GTATAAG35250.013.2875551
TAGTACA26250.013.1687424
Produced by FastQC (version 0.11.2)