FastQCFastQC Report
Fri 21 Jul 2017
HMN35AFXX_n02_mata2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN35AFXX_n02_mata2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4671619
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG12450.023.1531071
TAGGTAG19550.018.7876345
GTATTAG34000.017.1681981
ACTATGC32850.016.8768888
TATACGG25550.016.6306725
CTATGCA28500.015.6624839
CTGTGCG10200.015.5287439
TCTATAC36600.015.1545163
GTATAAT59250.015.0817561
GGTAGTC20250.014.9346097
TATACAG46300.014.7771715
GTATAGA34200.014.5392361
ACCGCGC5456.0218554E-714.5322638
GTATATA90400.014.5084681
TACACCG13500.014.4038525
TATACCG21700.014.2710985
CTCGGAG13150.014.2395525
TATATAC99550.013.8928833
GTATACG28250.013.7750731
CTAATAC40050.013.6692123
Produced by FastQC (version 0.11.2)