FastQCFastQC Report
Fri 21 Jul 2017
HMN35AFXX_n02_mata1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN35AFXX_n02_mata1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3470780
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG25550.018.0465661
TATACAG32350.016.2493085
TAGGACC10800.016.0031364
CTAATAC28500.015.668263
ACTATGC22550.015.0061318
TATATAC71550.014.9985963
TATACGG19800.014.9109385
GTATTAA38900.014.8165091
TACTATG29100.014.5978517
CTAGGAC7958.731149E-1114.4952873
CTAGTAC16950.014.4471833
TAGGTAG13000.014.4017845
GTAGGAC7556.202754E-1014.3092963
CTATGCA21200.014.2630399
GTATATA64950.014.1982991
GTATAGA26000.014.1319571
ACCGTGC6151.5353726E-714.0482928
TATATAG43150.014.0179595
TAGACAG15500.013.937215
TATACCG14050.013.8380125
Produced by FastQC (version 0.11.2)