FastQCFastQC Report
Fri 21 Jul 2017
HMN35AFXX_n02_mas4x58p28c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN35AFXX_n02_mas4x58p28c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4622844
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGC5103.6379788E-1119.7658218
GCGTCAG12500.019.0222471
GTCCCCG10850.016.590089
GTATAGA32200.016.1114461
TAGTCCG18000.015.6002059
GTATTAG34950.015.2560621
GTATATA87900.015.082971
TATACCG21550.015.0380945
TATACAG43150.015.0206675
TATAATA141850.014.675882
GTATAGG23700.014.5932081
AGTCCCG7907.8216544E-1114.5831018
TAGACAG18350.014.5208865
TATACGG25500.014.4031515
GTATATG50950.014.1420941
CCGTCGC9405.456968E-1213.78741559
GCCCGGC21000.013.7144669
AGACTCG11300.013.3834595
ATAGTCC31500.013.2578928
TACACCG14200.013.1859835
Produced by FastQC (version 0.11.2)