FastQCFastQC Report
Fri 21 Jul 2017
HMN35AFXX_n02_mas4x58p27g.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN35AFXX_n02_mas4x58p27g.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4582050
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTGC6900.017.738398
GCGTCAG12450.016.2043021
ATACGGT24450.015.9043026
TATACGG25550.015.7836085
TATACAG39450.015.698595
GTATAGA32100.015.4876581
CTATACT33950.015.4856784
GTATATA79400.015.4265961
TATACCG19500.015.1411365
GTATTAA50900.014.8632291
TAAGGAG29650.014.5725575
CCTAGGA7958.731149E-1114.49597552
TATAATA136400.014.2047142
CCGTGCA9650.014.17489
TAGACAG19350.014.1420335
CATATAC48000.014.1046483
TTATAGT49000.013.9628694
TACGGTG20650.013.9469117
TAAACCG16550.013.9239095
AGTTCCG27650.013.8035235
Produced by FastQC (version 0.11.2)