FastQCFastQC Report
Fri 21 Jul 2017
HMN35AFXX_n02_mas4x58p25e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN35AFXX_n02_mas4x58p25e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2910599
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAG30000.022.0850585
CTAGGGT7000.019.5496884
GTATATA58950.018.7038461
TAGAGCG7450.018.3637817
ATACAGG22550.017.883446
GTATAGA19600.017.280851
GTATTAG22700.017.1431731
CTGGGCG5051.36496965E-817.1075369
GTACTAG16850.017.1073741
TAGGACG5502.1118467E-917.024134
TATACGG14000.016.9753175
GAGCGCA7650.016.9398169
TAGGACT11750.016.5504634
CTAGGAC7051.2732926E-1116.3478223
TACAGGC14650.015.7281327
AGACCGC6006.708433E-915.6027696
GCCGACC11650.015.443404140-144
CTAGACT13250.015.2204124
TCGACCG2850.003504862815.1566769
TCTATAC21400.015.147043
Produced by FastQC (version 0.11.2)